Jandre2k3's Cardboard Flower Box - Test 4: ScrOG

Thanks Alaskan1... So, if I'm reading that right, you could, in theory, make an extraction from mmj, then distill it at different temperatures to seperate the different cannabanoids? Would that work and would they recondense if captured after vaporization? Just curious on that one, could be interesting and could lead to an easier way to medicate for different issues possibly.

Well, MY understanding of what I have read is in a vape pen THC is activated at 180 Degrees and the CBD is activated at 160 degrees.
To answer your questions though you need someone far smarter then myself
 
Thanks Alaskan1... So, if I'm reading that right, you could, in theory, make an extraction from mmj, then distill it at different temperatures to seperate the different cannabanoids? Would that work and would they recondense if captured after vaporization? Just curious on that one, could be interesting and could lead to an easier way to medicate for different issues possibly.

little more information for you folks

THC - Tetrahydrocannabinol, CBD - Cannabidiol and CNB - Cannabinol Exsist all within Marijuana, and are the key elements containing cannabinoids, which are the microscopic things that attach to recepters in your brain getting you high.
Here are the temperatures for activating these key elements.
At 50°C (122°F) THC-Acid decarboxylates as the water molecule held in the carbonate form evaporates. This activates the THC.
At 66°C (150°F) Cannabidiol (CBD) melts and starts to evaporate.
At 185°C (365°F) Cannibinol (CBN) boils.
At 200°C (392°F) THC boils. Clear vapor from a vaporizer.
500-700°C (932-1292°F) Is the combustion temperature for marijuana.
From this alone, we can see, that weed does not have to be on fire, (combustion process) for the THC/CBD/CNB to be doing its job.
At the temperatures required for combustion, or to have a cherry going,
500-700°C The key elements are being overheated and their chemical compounds are breaking down.
So, this evidence suggest that when you light up a blunt, or joint, or get cherry going in your bong bowl, you are wasting/destroying valuable THC.
When you light a blunt, the cherry burns slowly towards the vaccume, which is your lungs, the cherry of the blunt, (combusting weed, embers at the end)
is not what is getting you high, heat from the cherry is heating
but not combusting the weed behind the cherry to the temperatures needed to activate the thc.

So, to be more THC Effecient, one should try and to burn Joint/Blunt slowly, and knock large amounts of the cherry, leaving a small amount burning, just enough to Activate the weed behind it. This theory also proves that blunts get you higher than rolling papers.
The Evidence: Tobacco, which is found in blunt wraps, burns slower, and at a lower consistent temperature, than the gums found in rolling paper. A lower temperature, with a slower burn means less of the weed is combusting, which means less THC waste. Joints burn too fast and too hot, so i recommend only using them when you dont have a blunt available and when you not home where you can use glass peices.

But this same concept applies to bowls, and bongs....
Holding a lighter on the bowl of a bong or pipe, is just wasting weed, because you are combusting the whole bowl, you may seem like it gets you higher, but its just a head rush from a large amount of THC. But it will fade fast, and you will notice the high is not consistent.
The flame of a bic lighter, burns at 1970°C
That is far beyond the MAX required to activate cannabinoids (200°C)
So buy a soldering iron with adjustable temperature, and use at 180°C
Or Buy a vaporizer, which is the same concept, only heating the weed to a maximum required temperature.

When baking edibles, brownies, cookies, etc, the baking temperature is normally 350°F, which is around 180°C, which is perfect. And when eating marijuana certain forms of THC which cannot be acquired through smoking are activated in the digestion process. So edibles are nearly 100% Efficient. But best of all, they keep you high for a long time, i have been stoned all day off a couple brownies. With the bong, i have to smoke repeatedly throughout the day. 7 grams of weed (Quarter Ounce) Goes a long way in a batch of 12 Brownies.

Smoking weed, is the least efficient form of use, invest in some tools to help
conserve and make the best of what you have, or bake it and eat it instead as a much cheaper solution
 
damn thats some good info, i smoke joints, its the way we smoke it over here, we very rarely smoke blunts, i dont know anyone who does, i do from time to time but only because i grow, where i am people make their buds last, an 1/8th can last someone most the day with rolling joints, a bit of cig tobaco in some rizla then sprinkled with bud, thats how everyone smokes over here, i dont know anyone who uses a vape, we use bongs,pipes and buckets for those who know what they are, all involve using lighters to ingite the bud before inhaling.

so i might buy a vape and give that a try, i take it you can control the temps with a vap to get the desired temp, great info ill give some of it a try and buy myself a decent vape,

so would them temps be the same for hash or does hash burn at different temps due to the way its mashed together,

one thing i did see on a video, i think it was one of the cannabis shows, it was some sort of bong but instead of using a lighter to light it they used a piece of hot metal, they put what they called ear wax in the end of the bong, (ear wax im not really sure what it is but i think its some sort of extraction process that gives you an ear wax looking product, it was actually called ear wax, but i dont know what it is or how to make it, i could google it i guess) then after putting it in the bong they heated some sort of metal then when it was at right temp they placed this hot metal into the bong and it instantly fried the ear wax so i guess this could be temp controlled also,

great info, if i could reps you again i would, ill remember to reps you next time it lets me.
 
One last thing before I part and this is a very long read.
Before I do paste it here I want to thank jandre for letting me hijack his thread and posting information on the wonderful untapped potential of CBD.

By Dr Hornby and research team..

Well we ran some preliminary experiments with the Volcano. I will report these
findings with a caution that they are merely pilot experiments, to find our way only.
Figure i put my foot in it by promising results, not thinking about the consequences that
the findings may bring. And now we're realizing that we may be opening up a whole new can of worms.

Firstly, we have known for some time that the Volcano does not deliver all of the THC, particularly at
the lower settings, of around 5 and 6. We know this since we use high-pressure liquid chromatography
(HPLC) to measure the amounts of the most abundant cannabinoids, that are present in the sample,
before and after vaporization. By measuring the THC, for example, left behind in the sample carcass,
we can determine the efficiency of the vaporizer setting, since we also know what we started
out with.

Since this is a study of heating cannabis we must consider a couple of things.
One is that THC, plus the other cannabinoids, are not active as they sit in nature.
For them to interact with the THC receptor, firstly they must be de-carboxylated or activated.
Meaning CO2, must be kicked off the parent molecules, that in the un de-carboxylated
form are acids, chemically speaking. De-carboxylation, in this case, is accomplished by heating,
which, in turn, excites the chemical bonds of the, for example, THC molecule causing it to release
carbon dioxide at the weakest bond. This, subsequently, activates the molecule, allowing receptor interaction.

If more heat is applied to the molecule, eventually it will reach its boiling point and enter the vapor state.
De-carboxylation occurs before evaporation and over a broader range of temperatures.
And is critical for understanding cannabinoid pharmacology. Therefore in vaporization we have a two-fold
process occurring that allows the medicine to be delivered. De-carboxylation, followed by evaporation.

When we run cannabinoids by HPLC we see the acids of CBD, CBN and THC, plus trace amounts
of these already de-carboxylated cannabinoids (alcohols as they're called, chemically). We normally total the acids with the alcohols to come up with total THC, CBD or CBN for the sample.

In the experiments discussed here we vaporized a cannabis sample that had already been shown
to contain an unusually high amount of THC, with relatively high CBD.
We ran three separate experiments at Volcano settings 6, 7.5 and 9. At each test, the same
weight of cannabis was placed in the volcano and two bags vapped for the same time period.
Following vaporization, the carcass (sample left behind), for each temperature setting was analyzed
for the three most abundant cannabinoids.

We will attempt to tabulate below our experimental results.


Volcano Setting THC remaining mg/g CBN remaining mg/g CBD remaining mg/g

6 260 0.05 0.6

7.5 245 0.05 0.6

9.0 75 0.05 0.15

With all HPLC analysis there is an error window associated with the quantification. This window is normally acceptable if it has a coefficient of variation of 15% or less. This is simply a statistical evaluation of the standard deviation or variation around the mean, divided by the mean and a percent value taken.
So with THC there is often an error window of plus or minus 2% around the measurement and for CBD and CBN
this is more like plus or minus 0.05%. To convert the above results to a percent value, simply divide by 10.

So, what do we learn from these results. Nothing about decarboxylation, since it's not presented here,
although much is gained from observing the chromatography (not shown), where a one to one decrease
of the cannabinoid acid and increase in the alcohol is seen. Nevertheless, in the table we are showing only
the de-carboxylated actives that are left behind.

As stated earlier, the cannabis sample used in this experiment tested particularly high in THC,
running at 260, + or - 40 mg/gram. As shown in the table, this did not change for setting 6 on the Volcano
and changed only slightly for setting 7.5. We only see a significant decrease in THC at setting 9.
These results also confirm earlier observations.

The boiling points of the three cannabinoids measured are as follows:

THC 200 C
CBD 190 C
CBN 185 C

We can see from our before and after sample, that CBN is virtually all gone at setting 6, leaving
only residual amounts. The THC, however, all remains at this setting. As does the CBD.
At setting 7.5 the THC is fully de-carboxylated, but still present to roughly 95% of its original value.
Interestingly, at this setting the CBD is only half de-carboxylated.

At setting 9, all three are fully de-carboxylated and have moved to the vapor state.
A small amount of CBD remains as does some THC.

We can conclude from these very preliminary experiments that with the Volcano at setting
6 all the CBN is gone along with the more volatile terpenes and aromatics. Yet at this
setting, although the THC and CBD are being activated they have still not received enough heat
to enter the vapor state. In addition, at setting 7.5, similar conditions hold, with the THC still
not evaporated. It's only at setting 9 that THC receives sufficient energy to become volatile.

We did another experiment to six bags and behold, all is gone.
THC acid, converted THC, CBN and CBD, all gone skyward.
This is at Volcano setting 7 and six bags were taken.
So between 2 bags, where virtually all the THC is still present,
and bag six the THC is being boiled off. To 2 bags it's all
terpenes and aromatics.

Suppose, we will have to narrow it down, to see if there is
one bag where the THC, fly's, at setting 7. Bet it's bag 4.
We'll see...
We ran another experiment with the Volcano. This time we collected 6 bgas at setting 6.
This just doesn't move the THC into the bag, only decarboxylating about 20% but not boiling
any off. Its aroma therapy at setting 6, very little, if any, active THC is delivered.
Of course at this setting you will be blowing off the terepenes and other lower molecular weight
aromatics, that do, indeed, have psychoactive properties. Incidently, these compounds, termed
the essential oils, have been banned, as long as the plant. It's my notion that the euphoric part
of the cannabis experience is a result of the essential oils and not THC.

Finally, i must remind that these are merely preliminary experiments, used to guide the way
to collecting more confirmatory data. All experiments of this type must be repeated a number
of times, with means and standard deviations from the mean, determined.

More experiments with the Volcano, taking samples from various temperature settings and bag numbers. In all cases we are measuring the amount of the most abundant cannabinoids in a sample taken from the left-overs in the vaporization chamber. Since we know the mg/g amounts of THC, CBD and CBN in the starting material, we can determine how much of each cannabinoid has been "blown off", at what temperature setting and what bag number. I believe that earlier i stated that all of the active THC is not delivered until bag 4 at setting 7.5.

In this set of experiments samples were prepared by a member assistant, according to a defined method.
Here we investigated three other temperature settings of the Volcano: level 3, level 4 and level 8. At each
setting 4 or more bags were collected.

The sample used was a Kush with a THC level of roughly 150 mg/g. CBD and CBN both in the 3 mg/g range.
These sort of values are very common here in BC and the profile very typical of BC cannabis.

At level 3 we see efficient decarboxylation, so we know our sample is seeing heat, but not enough to send the THC skyward. This level is a good way of making an oral prep, from the carcass, since at bag 3 all of the THC is activated. But you would have to wait until at least bag 3 to get better than 90% of the THC in the activated form.

Level 4 experiments were conducted the same as for level 3. With the same amount of cannabis (0.3 g) being processed and bags collected in the same time period. Level 4 is no more exciting in terms of lifting the THC to the vapor state and is simply a continuation of efficient decarboxylation of the THC-A to active delta-9 THC.
Once again a bag 3 greater than 90% of the THC is activated, and less than 1%, blown off. Therefore breathing the vapors collected at bag 3, level 4, is at best aroma therapy, with very little active THC being delivered.

Now we go to level 8. Immediately, at bag 1 all the THC is decarboxylated and roughly two thirds delivered.
And at bag 3 80% of the activated THC is gone. So once again we make the observation that a level setting of 7.5, or above, is required for 3 bags to deliver activated THC. Lower settings, indeed deliver active medicine in the form of terpenes and aromatics, essential oils long know for therapeutic properties. I have often thought this is where the euphoric experience of cannabis lies in these, also prohibited, oils.
I find level 3-5 works good in the morning and in the evening I like level 9
After we ran the experiments we found the most members had great results burning on level 7
People with chronic pain back pain like level 9
 
awesome info A1 i love the important work your doing,i only wish i lived in a place where i could be involved in such sciences, my primary goal is breeding unique and powerful medicinal strains of cannabis, but this kind of testing and documentation will at some point become paramount to furthering my objective, *sigh * at some point im going to have to leave my cozy,sleepy little non cannabis friendly community,non friendly equates to alot of ignorance and lack of attention,a good thing for under radar style, despite being legal mmj auth to cultivate card holder i desire high privacy & security, im that way by nature if all i was doing was growing facial hair & playing blops2 i would still want my privacy,lol, im convinced cbd and some of the other various components are going to be "discovered" as having powerful medicial value, this is why whole plant use is proven to be more effective than single cannabinoid preperations, :thumb:
 
One of the tests we plan on doing soon it to grind up all the high CBD stalks left once the plant is harvested and grind the stalks into powder and have the powder tested to see what CBD it holds. We are also going to take some of that powder and turn it into BHO and have that tested as well. Who knows what we might find.
BID, you need to move to a mmj friendly state like Washington my friend.........just a suggestion, drag Jandre along with you lol
 
Yeah, it does seem less stressful here with the MMJ. I love Wa :) And I agree BID, I do think they're going to "discover" more and more medical benefits to the various cannabinoids over the coming years... also agree that I want to play the breeding game, aiming first at strains for me and my girl, then targeting others down the road.

Alaskan1 - Any idea on when y'all will be running that test with the high CBD stems testing? Will you be posting the results?
 
Yeah, it does seem less stressful here with the MMJ. I love Wa :) And I agree BID, I do think they're going to "discover" more and more medical benefits to the various cannabinoids over the coming years... also agree that I want to play the breeding game, aiming first at strains for me and my girl, then targeting others down the road.

Alaskan1 - Any idea on when y'all will be running that test with the high CBD stems testing? Will you be posting the results?

Should be a month or so and yes, I'll post the results
 
its a bit annoying when a company in the UK can grow tonnes of high grade cannabis for medical purposes and producing a spray called sativex, surely if the uk government can allow a permit for a company to grow then surely its only a matter of time before patients can legally grow their own meds,

i use to take many types of sleeping tablets and all made me feel like crap the next day, gave me the shakes and if anything happened in the night id be screwed, the one lot of meds i was on made me paralysed from the waste down, i woke up in the night and went to get out of bed and collapsed on the floor, couldnt move my legs at all so ended up sleeping on the floor, so stopped taking prescription drugs, when i was a lot younger i smoked cannabis and always slept well and ate well, so a few years ago i purchased some bud and it solved my sleep problems, it helped me put weight back on to a stage where im pretty happy with the way i look and feel about myself, i just need a good daytime smoke to help with my ibs pain and id be a very happy man indeed,

but with all these medical growing going on and new strains been bred with the cbd % im pretty sure its only a matter of time before i can get the meds that will make my day to day life a whole lot easier,

alaskan1 and jandre and doing a really good deed working with these strains, im not an expert grower far from it but if i had a way of testing what i grow then id be more than happy to spend my time trying to produce a high% strain, so its down to alaskan and jandre and the others who are trying to perfect their craft that we might actually end up with some perfect meds that solve a lot of problems for a lot of patients,

hats off to you gents, great work
 
One last thing before I part and this is a very long read.
Before I do paste it here I want to thank jandre for letting me hijack his thread and posting information on the wonderful untapped potential of CBD.

By Dr Hornby and research team..

Well we ran some preliminary experiments with the Volcano. I will report these
findings with a caution that they are merely pilot experiments, to find our way only.
Figure i put my foot in it by promising results, not thinking about the consequences that
the findings may bring. And now we're realizing that we may be opening up a whole new can of worms.

Firstly, we have known for some time that the Volcano does not deliver all of the THC, particularly at
the lower settings, of around 5 and 6. We know this since we use high-pressure liquid chromatography
(HPLC) to measure the amounts of the most abundant cannabinoids, that are present in the sample,
before and after vaporization. By measuring the THC, for example, left behind in the sample carcass,
we can determine the efficiency of the vaporizer setting, since we also know what we started
out with.

Since this is a study of heating cannabis we must consider a couple of things.
One is that THC, plus the other cannabinoids, are not active as they sit in nature.
For them to interact with the THC receptor, firstly they must be de-carboxylated or activated.
Meaning CO2, must be kicked off the parent molecules, that in the un de-carboxylated
form are acids, chemically speaking. De-carboxylation, in this case, is accomplished by heating,
which, in turn, excites the chemical bonds of the, for example, THC molecule causing it to release
carbon dioxide at the weakest bond. This, subsequently, activates the molecule, allowing receptor interaction.
<snip>
the Carboxyl group is not CO2.... it's CO2 with an extra hydrogen atom... represented by COOH



The boiling points of the three cannabinoids measured are as follows:

THC 200 C
CBD 190 C
CBN 185 C

We can see from our before and after sample, that CBN is virtually all gone at setting 6, leaving
only residual amounts. The THC, however, all remains at this setting. As does the CBD.
At setting 7.5 the THC is fully de-carboxylated, but still present to roughly 95% of its original value.
Interestingly, at this setting the CBD is only half de-carboxylated.

At setting 9, all three are fully de-carboxylated and have moved to the vapor state.
A small amount of CBD remains as does some THC.

We can conclude from these very preliminary experiments that with the Volcano at setting
6 all the CBN is gone along with the more volatile terpenes and aromatics. Yet at this
setting, although the THC and CBD are being activated they have still not received enough heat
to enter the vapor state. In addition, at setting 7.5, similar conditions hold, with the THC still
not evaporated. It's only at setting 9 that THC receives sufficient energy to become volatile.

<snip>


So what the above says to me, is set it to 185 to vape off the CBN... once that's cleared, crank it up to 184 and enjoy the healing, without the spaciness.
 
alaska1 im in a mmj state and a legal cardholding grower that abides by the rules, but that does not mean my community is friendly, its goofy to grow in az you have to live 25 miles from a dispensary, so to grow i have to live in the sticks, and the bright side is its highly unlikely there will ever be one close enough to me unless they build one in the sticks lol but it makes access to many things a royal pain to get to id have to drive for at least 2 hours to get to a disp. im all mail & the few ppl i know, but the closest is an hour! so yeah its bittersweet
 
there are no disp in Hawaii
they have mmj and expect law abiding cardholders to go their meds off the local dope dealers
how does that make sense
and when you actually ask the doctor or nurse when you do get a card they tell you you need to know someone or friends or whatever they cant legally help once you get the card your on your own here
which sucks cause some of the main people who get these cards legitimately are dying and don't have time to grow stuff, like my dad
so you just gotta buy whats around, quality depends on who you know getting specific strains are always hard when you have to buy from people who don't have the strains people needs or want require whatever
meh just my 2 cent rant sorry
 
BID, you need to move to a mmj friendly state like Washington my friend.........just a suggestion, drag Jandre along with you lol

Yeah, it does seem less stressful here with the MMJ. I love Wa :)

:high-five::slide::yahoo::yummy::ganjamon:

WA rocks! I'm pretty excited to check out the farmer's market. Wish the one in Spokane could get going again. Seattle is fffaaaaaaaAAAAAAAAAAaaaaaaarrrrrrr from the NE corner of the state. :blalol:
 
Yeah, we're planning on checking out the Olympia MMJ farmers market in the next couple of months. I just keep it put off a bit until we've got out tents and lights, because it'll be hard to not buy something and I tend to be the over-responsible one, i.e. "it would be a waste to go and buy stuff now when you have a tent and lights to purchase so you aren't forced to spend a lot of money at a dispensary each month", so we're waiting to check it out. I do think the concept is awesome though :)

We hit the regular farmers market a couple times a month or more for groceries.
 
I avoid the main grocery stores as much as I can. We get most of our food from the farmer's markets and out of our garden. Since we started doing this, we both notice that overall we don't feel as bad, unless we decide to "splurge" on those things we don't eat anymore, then we feel like crap for a while afterwards.
 
Okie Dokie, when the lights go off at 5am they will be on the 12/12 cycle from the GLR they were on. They have almost completely filled the screen for this ScrOG test, so I feel confident, watching how they grew from seed last time, that there will be a lot more growth still, but the majority of the screen is already. I'll have pics a little later- my camera doesn't like the flicker from my old magnetic ballast. so I'll have some pics after the lights go off with a tiny CFL. Meh, ya gotta do what ya gotta do.
 
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