Vancouver Island 2022 Organic Grow

[Mycelium Farmer]

But I would think the mother either would or would not already have that resistance. I'm not sure innoculating a plant works the same way it does with our immune system. Hopefully the breeders in the crowd can weigh in.

Great example! on point @Azimuth

 

[Danishoes21]

But I would think the mother either would or would not already have that resistance. I'm not sure innoculating a plant works the same way it does with our immune system. Hopefully the breeders in the crowd can weigh in.

I don't think introducing a plant to PM gives it the ability to combat it just like that.... otherwise cannabis wouldn't have a PM issue by now.... but there is defenitely something to do with genetics and PM resistance, considering certain strains are more susceptible than others.

Maybe introducing PM to the plant can give you an idea of what those certain genetics can handle PM wise though??

I just started reading this.

Have a look at it and let me know what you think. it’s long but has many examples of fighting PM on many different crops.

Salicylic acid - Wikipedia

en.m.wikipedia.org

Plant Defense Mechanisms Are Activated during Biotrophic and Necrotrophic Development of Colletotricum graminicola in Maize - PMC

Hemibiotrophic plant pathogens first establish a biotrophic interaction with the host plant and later switch to a destructive necrotrophic lifestyle. Studies of biotrophic pathogens have shown that they actively suppress plant defenses after an ...

www.ncbi.nlm.nih.gov

pathogen erysiphe cichoracearum: Topics by Science.gov

Frontiers | Editorial: Biotrophic Plant-Microbe Interactions

Biotrophs and other partnersOrganisms inhabit the biosphere not as isolated entities: they interact with others. These may be individuals of the same species...

www.frontiersin.org

There is lots there to digest.

 

[Danishoes21]

Great example! on point @Azimuth

“Pathogen-induced elicitin production in transgenic tobacco generates a hypersensitive response and nonspecific disease resistance.

PubMed Central

Keller, H; Pamboukdjian, N; Ponchet, M; Poupet, A; Delon, R; Verrier, J L; Roby, D; Ricci, P

1999-01-01

The rapid and effective activation of disease resistance responses is essential for plant defense against pathogen attack. These responses are initiated when pathogen-derived molecules (elicitors) are recognized by the host. We have developed a strategy for creating novel disease resistance traits whereby transgenic plants respond to infection by a virulent pathogen with the production of an elicitor. To this end, we generated transgenic tobacco plants harboring a fusion between the pathogen-inducible tobacco hsr 203J gene promoter and a Phytophthora cryptogea gene encoding the highly active elicitor cryptogein. Under noninduced conditions, the transgene was silent, and no cryptogein could be detected in the transgenic plants. In contrast, infection by the virulent fungus P. parasitica var nicotianae stimulated cryptogein production that coincided with the fast induction of several defense genes at and around the infection sites. Induced elicitor production resulted in a localized necrosis that resembled a P. cryptogea-induced hypersensitive response and that restricted further growth of the pathogen. The transgenic plants displayed enhanced resistance to fungal pathogens that were unrelated to Phytophthora species, such as Thielaviopsis basicola, Erysiphe cichoracearum, and Botrytis cinerea. Thus, broad-spectrum disease resistance of a plant can be generated without the constitutive synthesis of a transgene product.”

Resistance Against Biotrophic Pathogens​

The plant immune system evolved to cope also with biotrophic pathogens. A key initial event of immunity is the perception of pathogen-derived molecules (“patterns”) by membrane-resident receptors (often dubbed pattern recognition receptors; Raaymakers and van den Ackerveken; Rajaraman et al.). A second layer of plant defense rests on the direct or indirect recognition of secreted pathogen effectors (“avirulence proteins”; Bourras et al.) by typically cytoplasmic immune sensors (“resistance proteins”; also termed nucleotide binding-oligomerisation domain (NOD)-like receptors) that usually confer isolate-specific resistance (Williams et al.). Execution of the actual defense response often involves re-organization of the host cytoskeleton (Tang et al.) and secretory activity (Xu et al.; Liu et al.). In addition, phytohormone signaling (Di et al.; Guerreiro et al.) and other plant components may contribute to resistance (Liu et al.), or immunity might be conditioned by the absence of essential host factors (Zheng et al.).

 

[Danishoes21]

The Clones
Had the time and the curiosity to see how things are going with this bunch of ladies in the bush. Well pretty good, lots and lots is happening, seeing a lot more pistils for the Personal Sativa clones but very far behind. Xenomoprh clones well making buds and doing it good, small plant but good enough size buds and starting to some sugar on those sugar leaves.
And I might just get what I asked for… turns out that the big pheno in the pot the one that is not a clone (formally know as the isolated one) she seems like a he. It’s still to soon to tell but she might bring that miracle of making seeds. It would be sooooo good if it’s a male. I could even pollinate the mothers. I’m excited about seeds. Just need to be patient and keep stressing the plants.

I’m trying a biological stress at the moment and I will get to explain that. Biothropic pathogen (powdery mildew) stress; basically I sprayed the clones with a another plant (common plantain) that had Powdery mildew, I took this organic matter and stuffed a jar and added molasses and water and it’s been in anaerobic fermentation for days. I added a part of this liquid to my neem foliar spray and some fern tea.

There isn’t much that can be found on the matter since no one in there right mind would wanna spray PM particles on to their cannabis. Let’s see what happens.

 

[Danishoes21]

this is the fern tea at a little over a week. Just wanted to share how it looks and the amount I used today.

This last image is the Common plantain with Powdery Mildew anaerobic brew the Color is different and the floating dots are seeds. I used a part of this liquid for the roots watering and for the foliar spray today

 

[Bill284]

this is the fern tea at a little over a week. Just wanted to share how it looks and the amount I used today.

This last image is the Common plantain with Powdery Mildew anaerobic brew the Color is different and the floating dots are seeds. I used a part of this liquid for the roots watering and for the foliar spray today

Thanks Ds21 that's very informative.
Hope I can remember half at least.
Good work Amigo.
Take care.




Stay safe
Bill284

 

[greenjeans]

this is the fern tea at a little over a week.

I made a batch starting Sept. 1 - I'll wait and see what yours does - no sense in both of us going down in flames!!!!

 

[Danishoes21]

I made a batch starting Sept. 1 - I'll wait and see what yours does - no sense in both of us going down in flames!!!!

I’ll take one for the gang.

 

[scottreid1966]

I’ll take one for the gang.

I have ferns to make tea. Just not sure what you mean by plantains. Sounds like those little bananas. I do have banana peals soaking in water though. Thought that I would use it to regenerate my outside soil this fall. I screwed up on the egg-shells and didn't cook the membranes off. Will redo.
I would really like to do organic root enhancers. That "Roots Excellerator Gold" that Bill uses is crazy expensive for such a little bottle. Won't really know the results untill my "phase II" goes to flower in a few weeks.
Between you and @Azimuth , I will learn.
Appreciate all the great 411 when it comes to organic grows.

Cheers!

 

[Danishoes21]

I have ferns to make tea. Just not sure what you mean by plantains. Sounds like those little bananas. I do have banana peals soaking in water though. Thought that I would use it to regenerate my outside soil this fall. I screwed up on the egg-shells and didn't cook the membranes off. Will redo.
I would really like to do organic root enhancers. That "Roots Excellerator Gold" that Bill uses is crazy expensive for such a little bottle. Won't really know the results untill my "phase II" goes to flower in a few weeks.
Between you and @Azimuth , I will learn.
Appreciate all the great 411 when it comes to organic grows.

Cheers!

Common plantain is this

Plantago major - Wikipedia

en.m.wikipedia.org

The minerals in ferns look something like this:
Table4. Mineral composition of N. cordifolia Leaflets
Mineral element Quantity (mg/100g)
Sodium 57.45±0.45
Potassium 1985.0±5.00
Phosphorus 207.80±2.20
Magnesium 142.0±2.00
Calcium 541.50±1.50
Iron 36.30±0.30
Zinc 6.15±0.50
Manganese 2.45±0.11
Copper 0.33±0.03

The reason behind I wanted to do anaerobic brew of ferns is because of that chart up there.

For the root enhancers or microbial enhancement and propagation, @Azimuth is proposing a measurable and proven to work Jadam and natural Korean farming methods and techniques, we share same views on very key and important factors like the impact of anaerobic teas and everything we have been back and forth. I share lots of common ground with these natural farming techniques and ways to make natural ready plant food, but to tell you the truth Im just trying many different things to see the effects, most things I have never tried before like fern tea, or horsetail tea for foliar spray.

The whole idea behind what I do is to save money and use only natural or as natural as possible ingredients and derivatives. A lot of the things I am trying on this grow are experimental, like using common plantain (see the link above), that had Powdery mildew on it and I started to do a jar of anaerobic fermentation to later use it to foliar spray my clones with that to see if they build immunity against the PM. Things like this are unorthodox and could cause misunderstanding if other people want to use it.

Hope this helps

 

[Azimuth]

Common plantain is this

I got some seeds of what I think is called upright plantain to grow in my garden. It will provide much more product at harvest per square foot than the common version which I currently use. I'll sow it in a few weeks just before frost to (hopefully) have it germinate in the spring.

Good luck with your experiment with the pm.

 

[Danishoes21]

I got some seeds of what I think is called upright plantain to grow in my garden. It will provide much more product at harvest per square foot than the common version which I currently use. I'll sow it in a few weeks just before frost to (hopefully) have it germinate in the spring.

Good luck with your experiment with the pm.

It’s on, sprayed on my last visit.
Whats your take on salicylic acid (from willow leaves and bark) to use against PM and rust ?

I have already found willow trees around here and plan on harvesting organic matter from it and anaerobic with it…




The article on Maize and the self defence mechanism of producing H2O2 to defend itself…?

“
One important feature that is induced once a pathogen is recognized by the host plant is an army of defense mechanisms that counteract the attack. Plants depend on several defense mechanisms to attack microbial intruders that threaten their physiology. The production of reactive oxygen species (ROS), primarily in the form of superoxide and hydrogen peroxide (H2O2), at the penetration site is one of the most rapid plant defense reactions after pathogen attack (Apostol et al., 1989). Apoplastic peroxidases can also use the H2O2 in reactions involved in the synthesis of lignin and other phenolic compounds that act as additional antimicrobial barriers (Torres and Dangl, 2005).

Many plants respond to fungal attack with the accumulation of ROS as a means to arrest fungal growth. ROS production was demonstrated to be one of the earliest cytologically detectable responses to restrict infection by various fungal species (Mellersh et al., 2002). Mellersh et al. (2002) described a comprehensive study on the relevance of plant defense mechanisms for fungal penetration in plant-fungus interactions. That article highlighted the importance of H2O2, superoxide, and phenolic compounds to prevent penetration and restrict fungal growth. In contrast, fungi have developed biochemical mechanisms to overcome elevated levels of intracellular ROS (van Kan, 2006). The methods employed to control ROS can include detoxification or scavenging (Rolke et al., 2004; Lev et al., 2005; Voegele et al., 2005; Molina and Kahmann, 2007). In addition, recent reports describe an important role of ROS produced by the fungus in virulence and development in some fungi (Heller and Tudzynski, 2011). These varying responses during the plant’s interaction with different fungal pathogens prompted us to further study and characterize the cytological and molecular aspects of the infection of maize leaves by the hemibiotrophic pathogen C. graminicola.”

 

[scottreid1966]

It’s on, sprayed on my last visit.
Whats your take on salicylic acid (from willow leaves and bark) to use against PM and rust ?

I have already found willow trees around here and plan on harvesting organic matter from it and anaerobic with it…




The article on Maize and the self defence mechanism of producing H2O2 to defend itself…?

“
One important feature that is induced once a pathogen is recognized by the host plant is an army of defense mechanisms that counteract the attack. Plants depend on several defense mechanisms to attack microbial intruders that threaten their physiology. The production of reactive oxygen species (ROS), primarily in the form of superoxide and hydrogen peroxide (H2O2), at the penetration site is one of the most rapid plant defense reactions after pathogen attack (Apostol et al., 1989). Apoplastic peroxidases can also use the H2O2 in reactions involved in the synthesis of lignin and other phenolic compounds that act as additional antimicrobial barriers (Torres and Dangl, 2005).

Many plants respond to fungal attack with the accumulation of ROS as a means to arrest fungal growth. ROS production was demonstrated to be one of the earliest cytologically detectable responses to restrict infection by various fungal species (Mellersh et al., 2002). Mellersh et al. (2002) described a comprehensive study on the relevance of plant defense mechanisms for fungal penetration in plant-fungus interactions. That article highlighted the importance of H2O2, superoxide, and phenolic compounds to prevent penetration and restrict fungal growth. In contrast, fungi have developed biochemical mechanisms to overcome elevated levels of intracellular ROS (van Kan, 2006). The methods employed to control ROS can include detoxification or scavenging (Rolke et al., 2004; Lev et al., 2005; Voegele et al., 2005; Molina and Kahmann, 2007). In addition, recent reports describe an important role of ROS produced by the fungus in virulence and development in some fungi (Heller and Tudzynski, 2011). These varying responses during the plant’s interaction with different fungal pathogens prompted us to further study and characterize the cytological and molecular aspects of the infection of maize leaves by the hemibiotrophic pathogen C. graminicola.”

Thanks, bro.
I've been trying to keep up with the thread. Took a few notes, just made my second batch of Dandylion tea. So much to learn so I save them for future study. I could use some natural root enhancer. That Excellerator Gold costs a small fortune..

 

[Danishoes21]

This is good information

Improvement of the antioxidant activity, phytochemicals, and cannabinoid compounds of Cannabis sativa by salicylic acid elicitor - PMC

Recently, due to the valuable and high level of phytochemical compounds such as cannabinoids and other secondary metabolites, the cultivation of Cannabis sativa has increased in the world. The current study was conducted to evaluate the potential ...

www.ncbi.nlm.nih.gov

Save the read if you want to jump in the results and conclusion:

RESULT AND DISCUSSION​

3.1. Total phenolic content (TPC) and total flavonoid content (TFC)​

The results demonstrated that total phenolic and total flavonoid contents in C. sativahave been affected by various concentrations of salicylic acid (p < .01). By applying different SA concentrations as foliar treatment, the TPC and TFC values in C. sativa plant increased from 7.59 to 23.06 mg GAE g−1 DW and from 3.38 to 11.28 mg Que g−1 DW, which are presented in Figure 1. The highest TFC and TPC were obtained in 1 M treatment, whereas the lowest TFC and TPC were obtained in control plants.

FIGURE 1
Effect of different concentrations of salicylic acid on total phenol content (TPC) and total flavonoid content (TFC) of C. sativa
Sánchez‐Chávez et al. (2011) reported an increase in total phenolic and flavonoid contents in Capsicum annuum fruits with exogenous salicylic acid spray applications, whereas Vázquez‐Díaz et al. (2016) demonstrated a greater concentration of phenolic compounds in Solanum lycopersicom due to the increase in salicylic acid doses. The results of the present study are in agreement with the latter. Phenylalanine ammonium lyase (PAL), as the first committed enzyme in the phenylpropanoid metabolism, plays a key role in the biosynthesis of phenolic compounds in herbs (Ejtahed et al., 2015). Saba et al. (2012) indicated a significant positive correlation between content of polyphenols and activity of PAL enzyme. Moreover, it was found that salicylic acid stimulates the accumulation of hydrogen peroxide (H2O2), which induces a higher activity of PAL enzyme, responsible for the biosynthesis of polyphenols (Hao et al., 2014). The result of present study revealed that salicylic acid application achieved a positive effect on phenolic compounds in Cannabis. Probably, exogenous application of salicylic acid regulates the production of polyphenols via stimulating PAL enzyme activity. Moreover, Cannabis sprayed with salicylic acid indicated significantly greater amounts of total flavonoids than that in control plants. Chalcone synthase is considered to be the key enzyme for flavonoid biosynthesis of plants. According to previous studies on other herbs, the exogenous application of SA led to enhanced chalcone synthase activity (Gazdik et al., 2008).
Furthermore, application of SA regulates the jasmonate signaling pathway, which in turn mediates the elicitor‐induced accumulation of flavonoid compounds (Khalili et al., 2009). Sun et al. (2016) reported that application of salicylic acid on Artemisia vulgaris causes alterations in values of antioxidants, phenolics and in the activity level of genes associated with flavonoid synthesis.

 

[Azimuth]

Whats your take on salicylic acid (from willow leaves and bark) to use against PM and rust ?

No experience with it for those. I have a knf willow extract I use for a pre-cloning soak along with seaweed extract.

The article on Maize and the self defence mechanism of producing H2O2 to defend itself…?

Will be cool if your pm experiment shows potential.

 

[Danishoes21]

No experience with it for those. I have a knf willow extract I use for a pre-cloning soak along with seaweed extract.


Will be cool if your pm experiment shows potential.

It would be cool to get a noticeable difference either good or bad (preferably good lol)

I’m thinking of visiting the clones Friday and start the willow jar this weekend, possibly ready to use in one week?

Do you have a suggested dosage or measurements from KNF and the willow extract ?

 

[Azimuth]

Do you have a suggested dosage or measurements from KNF and the willow extract ?

Most of my knf extracts are used at 1:750 although 1:1,000 is the actual recommended dilution.

 

[Danishoes21]

Most of my knf extracts are used at 1:750 although 1:1,000 is the actual recommended dilution.

I hate to ask that many questions; I’m mostly a figure it myself kinda person BUT hehe; how much organic matter is suggested to collect to make each extract? I have never weighed the amount of organic matter I grab from any of my teas or anything… just eye ball it all the time.

I’m gonna grab a hefty amount of leaf and parts of bark from the willow (bark I will try to pulverize before putting in the jar).

Anyway can’t wait to see the plants and spray them again. Starting to get cold around here and the morning condensation is getting bigger and bigger, this is about the time when fungi starts the magic of destruction. On a happier note it’s chanterelle season all over.

 

[Azimuth]

I hate to ask that many questions; I’m mostly a figure it myself kinda person BUT hehe; how much organic matter is suggested to collect to make each extract? I have never weighed the amount of organic matter I grab from any of my teas or anything… just eye ball it all the time.

I’m gonna grab a hefty amount of leaf and parts of bark from the willow (bark I will try to pulverize before putting in the jar).

Anyway can’t wait to see the plants and spray them again. Starting to get cold around here and the morning condensation is getting bigger and bigger, this is about the time when fungi starts the magic of destruction. On a happier note it’s chanterelle season all over.

Ask away. No issues on my end.

The knf sugar extractions are basically equal amounts, by weight, of sugar and plant material mixed thoroughly so the sugar coats the plant material well. No water. Fill the jar/container 2/3rds full, cover with a breathable lid and sert aside. In 7-10 days you'll have most of what you're likely to get so you can drain and screen it then. Plant material can then go in your compost pile.

The liquid will be a dark brown color, thicker than water but not quite syrupy and will depend on the amount of material you use, but it's not all that much. It's quite concentrated though and may cause pH issues if you use too many at a time. I often use several together like seaweed, comfrey, fish and wca. I haven't had issues, but something to be aware of.

 

[scottreid1966]

Common plantain is this

Plantago major - Wikipedia

en.m.wikipedia.org

That was an awesome link, man!

I have those plantains around here. That wikipedia info was very informatine, put the link in my cannabis folder.

My journal may only have 3 followers, but am fortunate to have quality over quantity.

Thanks, guys! YOU'S DA BEST!

Cheers!