Since this diary had an unusual twist of faith; I will be inoculating a bag of Uncle Ben's Whole Grain Brown Rice. Until I get some more grain jars. So let's get this show on the road.
I now have the supplies I need to inoculate the rice bag. Which included a sterile work area.
Materials Needed
- One or more bags of pre-cooked plain brown rice, any brand, but people obviously almost always use Uncle Ben's whole Grain Brown Rice
- A syringe of liquid spore solution. @Spore Genetics Nameless spores
- Alcohol and a clean cloth
- Micropore tape
- Scissors
- A lighter or other flame-source
- Rubber Gloves
Day 1: Inoculation
My best practice is to perform the inoculation process in front of a flow hood or in a still-air box. But for this try I will do a straight inoculation into the bag.
Consequently, I set up in a room with little to no airflow and made sure to spray an aerosol disinfectant.
Inoculation Steps:
Day 1: Inoculation
To perform the inoculation process in front of a flow hood or in a still-air box. But for this try I will do a straight inoculation into the bag.
Consequently, I set up in a room with little to no airflow and made sure to spray an aerosol disinfectant.
Inoculation Steps:
1. Wipe down all surfaces, materials, and utensils with 70% isopropanol alcohol.
2. Flame sterilize your needle until red hot. Allow to cool for a few seconds, then poke a hole through the bag near the top, just large enough to fit your needle in the bag.
3. Remove the cap from your spore syringe and flame sterilize until red hot. Allow the needle to cool, then insert in the small hole and inject 1cc of spore solution into the bag.
4. Replace the needle cap and set it aside.
5. Using micropore tape, re-seal the cut you made in the bag.
6. Repeat this process making sure to wipe down all utensils, bags, and flame sterilize your needle with every bag. Once finished with inoculation we set our bags in a dark and dry place for colonization. The ideal temperature for colonization is around 78° F.
7. Check your bags every couple of days for signs of contamination. A contaminated bag may show signs of mold or put off a foul smell. If contamination is suspected, throw the bags away and try again.
8. After 5 to 7 days, I should see mycelium beginning to form through the clear window at the bottom of the bag.
Day 13:
Time to break up
Upon checking my bag’s, it appeared that they were roughly 80% colonized. While only a little bit of mycelium is visible from the window at the bottom of the bag, the bags feel dense which indicates that the mycelium network has taken hold of the rice. Additionally, the bags smell wonderful! No signs of contamination!
To speed up the growth I will break up the mycelial mass and evenly distribute the colonized rice with the un-colonized. This will help speed up the colonization process, but you will want to wait until roughly 60% of the grain has been colonized before performing this step.
Based on my rate of colonization, I could do this around day 10.
Day 19: Spawn to Substrate
Note: I will provide a substrate formula soon.
That's all folk's.
Be safe, and grow well my friends,
Tok..