Rex's Perpetual Hydroponics, LED, 4x4 Tent Using TBC 10 Gal System: All Are Welcome, Come Learn & Help

Hey brother I hope all is good.
congratulations my friend. I totally missed this one lmao. :thumb:

Play safe and enjoy everyone
Thank you brother! Things are going good, I've never had to try to clean 100L of hydroton before...its been a little more time consuming then expected. The frequent thunderstorms have also slowed things down, just something about using a long hose next to a big metal shed during a thunderstorm that doesn't feel smart :hmmmm: :rofl: . But that task is almost done, & I've got new beans in the mail for the next grow :yahoo:
 
Thank you brother! Things are going good, I've never had to try to clean 100L of hydroton before...its been a little more time consuming then expected. The frequent thunderstorms have also slowed things down, just something about using a long hose next to a big metal shed during a thunderstorm that doesn't feel smart :hmmmm: :rofl: . But that task is almost done, & I've got new beans in the mail for the next grow :yahoo:
Always safety first brother. I’m glad you got shit on order. Let’s get this party started my friend. I wouldn’t miss it for the wold.
Play safe and enjoy my brother
 
So another harvest happened!


The little stunted auto has been harvested. Took all of 5 min to chop and give a light rinse. She'll go into the fridge soon for drying.

This journal will stay open. And I will be returning to reboot it after my next Sponsored journal is completed.

Everyone is welcome to come over and join in :green_heart:


Meanwhile feel free to post and pictures, questions or anything hydroponic related in here! Thanks everybody for following along!
 
Congrats! I've been taking a break lately,while my last harvest cures....but super excited to have put down some girl scouts today!!! Ehhh, I showed the hubby ur harvest and he was like damnnnn lol. I said I know right, that's one of the guys that help me run our last crop!
Thanks Mcdoolit, you did amazing on your run, and all the work!
Have you started a journal for your girl scout cookies? Feel free to drop a link here if you do (or when ;)) or in my new journal!
 
Thanks Mcdoolit, you did amazing on your run, and all the work!
Have you started a journal for your girl scout cookies? Feel free to drop a link here if you do (or when ;)) or in my new journal!
I appreciate it! I always get nervous with journals. It feels like too much pressure lol.
I'm sure I'll post some pics and get a thread going once they start though. :D I'm sure it'll be soon and I'll be screaming at y'all for help! Lol
 
I appreciate it! I always get nervous with journals. It feels like too much pressure lol.
I'm sure I'll post some pics and get a thread going once they start though. :D I'm sure it'll be soon and I'll be screaming at y'all for help! Lol
Well just know that if you don't have a journal feel free to drop any questions dont be shy to tag me, or post in here (then anyone following here can chime in as well). No pressure either way!
 
Congrats on an awesome run mate. I'll pop over and grab a seat in the new journal.
:goodjob:
Thanks Vet! Appreciate having you along brother, and congratulations on your awesome harvest!
Rexer, Nice harvest, those buds are fat. Cheers
Thank you Cr8! They've been smoking quite well too. It's nice to be back on home growin :)
 
Hey everyone!

Been a while since I posted in this journal, but thought I would share this article I came across...lots of great little bits of good knowledge in it:

POLITICSSCIENCE & HEALTHCULTUREBUSINESSVIDEONEWSLETTERBILL TRACKINGABOUT MARIJUANA MOMENT.net




Scientists Create New Method To Store Marijuana Pollen On A Long-Term Basis

Published
1 year ago
on April 29, 2020
By
Kyle Jaeger



Researchers have developed a way to determine the viability of pollen germination in marijuana plants, as well as a simple method of storing cannabis pollen for long periods of time, according to a new study.

For marijuana cultivators, the results could prove useful, helping them avoid potentially costly mistakes in the process and ensuring consistency and quality in their crops across growing cycles.

Ensuring long-term storage of pollen is important for cultivators because of the central role it plays in generating seeds. Taking pollen from a male plant and rubbing it on the hairs of a female plant—typically about halfway through the flowering cycle—will enable the female plant to produce buds that contains seeds, which along with cloning is one way to propagate and maintain strains.

The study, which was partly funded by the Canadian government, has “several implications,” co-author Igor Kovalchuk told Marijuana Moment.

First, the team created an “assay to test viability of such pollen before the use for pollination.”

They accomplished that by modifying an existing method of assessing germination viability, using a liquid media instead of a solid medium, which “resulted in better image acquisition and quantification of germination,” according to the study.

Perhaps even more consequential for growers, however, is the development of a long-term storage system for cannabis pollen.

“We have provided an easy protocol for cryopreservation using desiccation combined with baked wheat flour and subsequent long-term storage of cannabis pollen in liquid nitrogen.”

“This one is big,” Kovalchuk said. “Our protocol allows nearly indefinite storage,” which is “valuable for maintaining large collection of genetics.”

To preserve the pollen, the researchers removed any moisture, added the result to baked whole wheat flour and preserving agents and then froze it in liquid nitrogen. When they removed the mixture from the liquid nitrogen and applied it to flowering female plants, it resulted in successful seed formation in all of the subjects.

“By using this method, we were able to maintain germination viability in liquid nitrogen after 4 months, suggesting potentially indefinite preservation of cannabis pollen.”

The team also discovered that pollen can be more or less viable at different stages of the flowering period. The optimal time to extract pollen seems to be during the mid-flowering stage. At that point, it retained “viability the longest with 22 percent of pollen grains successfully germinating after 21 days” of storage in a low-temperature environment.

“In conclusion, we have standardized a simple assay for quickly assessing pollen germination in Cannabis sativa,” the study states. “By using our [modified assay], we have demonstrated the loss of pollen viability over time when stored at 4 degrees Celsius, and suggested an optimal time during flower development for pollen collection to maximize longevity during storage.”

“Finally, we have provided an easy protocol for cryopreservation using desiccation combined with baked wheat flour and subsequent long-term storage of cannabis pollen in liquid nitrogen,” it concludes.

END

While not viable for the average grower, it was a great read.
 
I did not know you had a perpetual! Now I do. :)
The beginning of this journal was quite a mess. Lots of stumbles, and me kinda frustrated. Feel free to skip all of it, though towards the end har pics here and harvest numbers here are worthwhile looking at.
 
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