Lady Cannafan Shrooming Midwest Grow Kits & Spore Genetics - APE, Blue Meanie & More

Lady Cannafan said:
With the great products grown from Midwest Grow Kits, and Spore Genetics, I have been able to make spore prints and my own spore syringes.
I did two syringes this morning using Blue Meanie Spore prints. Two more will be done today using Tidal Wave prints.

I had to make my own distilled water yesterday, the grocery delivery gave me "smart Water" with electrolytes instead of the Distilled water jug I had ordered. I don't know if that electrolytes process affects it or not, so I opted to make my own.
Everything was sterilized, including the shot glass for the liquid. I heated the needle and the e-xacto knife blade up before putting them into the SAB.
This went well. I had two small spore prints and used one for each syringe.
The spores were scraped off the aluminum foil into the shot glass. Then 10ml distilled water drawn into the needle, and dispersed into the shot glass. Then I drew it back into the syringe and out again a couple of times just to be sure all the spores made it in there.

I did get a little air bubble in both syringes, but they are small. You can see the spores in the end of the syringe in the last pic.
Later I will be doing transfers to agar plates, probably using some of the syringes from Spore Genetics. I have some of the Blue Meanie and Tidal Wave left in those.

Spore syringes from spore prints.jpg

Spore syringes from spore prints.jpg


Spore syringes from spore prints-2.jpg

Spore syringes from spore prints-2.jpg


Spore syringes from spore prints-3.jpg

Spore syringes from spore prints-3.jpg

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Looks amazing in there
 
I've just put most of the cakes in the fruiting tub back into the final dunk. I only got a few off the Blue Meanies this time, and nothing on the APE again. Maybe the third time will be a charm. I've left the Tidal wave until tomorrow because there are a couple shrooms growing on them.
 
Nothing showing for AIO bag yet. It's day 9, so I have a few days to go in expectation of any signs of shrooms.
Today I am doing spore transfer to agar plates. The spores will be Tidal Wave.
I also have my own Agar made up and in the pressure cooker right now. That will be transferred to plastic condiment cups with lids that I had a ton of here.
That Philly Golden Teacher dude is awesome!
 
I'm getting mixed answers on this. Is it better to store the agar plates at room temperature or in the fridge?
I wound up making too much because I overshot the corn syrup when weighing things out. It worked out exactly double the batch. We'll see how many I wind up with, but I need to know what's the best way to store them.
 
Lady Cannafan said:
I'm getting mixed answers on this. Is it better to store the agar plates at room temperature or in the fridge?
I wound up making too much because I overshot the corn syrup when weighing things out. It worked out exactly double the batch. We'll see how many I wind up with, but I need to know what's the best way to store them.
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I keep mine in the fridge
 
Thank you @Ramblinrose1965 !
They are headed to the fridge now.

Here's my ingredients, and the pouring was done in the SAB after the jars were under 160 degrees.
I hope my brother is serious about growing shrooms, I have enough of these to supply both of us. There are 18 stacks of three. LOL I dripped a little. Had to do a clean up in aisle 4. Easy to clean once it cooled.
I sure hope I did this right!

Agar plates in condiiment containers.jpg

Agar plates in condiiment containers.jpg


Agar plates in condiiment containers-2.jpg

Agar plates in condiiment containers-2.jpg


Agar plates in condiiment containers-3.jpg

Agar plates in condiiment containers-3.jpg


Agar plates in condiiment containers-4.jpg

Agar plates in condiiment containers-4.jpg

 
Just to be clear LadCannafan....I refrigerate them after they have colonized with mycelium to store for long periods of time. The mycelium goes dormant once it hits the fridge.
 
Ramblinrose1965 said:
Just to be clear LadCannafan....I refrigerate them after they have colonized with mycelium to store for long periods of time. The mycelium goes dormant once it hits the fridge.
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I'm glad you mentioned that. :thanks: I haven't done any transfers to the cups yet, just the agar done up. They are all in the fridge right now.
 
UPDATE Shroom Room: 12-8-23

I got a nice surprise this morning in the Ecosphere house. One of the All In One Bags has the first mushroom growing. :yahoo:
I will have to look later at what day this is from injection, but it seemed like years. LOL This is the one I forgot to label when doing the inoculation.

I dunked all of the cakes for the third time, and this time put them in a shoebox with perlite and put those in the Ecosphere as well. I have a few fruits right now, and I noticed that the fuzzy feet are not near as bad as they were my first time around. I think the humidity in the Ecosphere is controlled more than the monotub for this.

I also had broken up the cakes of APE that took forever in the first jars for mycelium, and integrated them into my homemade mix.
Mycelium started showing day before yesterday, so I'm hoping for success.

I have one shoebox with what appears to be a lot of cobwebbing. That was from an All In One bag that I had broken up and mixed with my substrate. I read that you can spritz it with Hydrogen Peroxide and if it's cobwebbing it will melt away. That's exactly what it did. So, I put that shoebox in the monotub in a different room and will keep watching it.

Shrooms update 12-8-23-2.jpg

Shrooms update 12-8-23-2.jpg


Shrooms update 12-8-23.jpg

Shrooms update 12-8-23.jpg


Shrooms update 12-8-23-4.jpg

Shrooms update 12-8-23-4.jpg


Shrooms update 12-8-23-3.jpg

Shrooms update 12-8-23-3.jpg


Shrooms update 12-8-23-5.jpg

Shrooms update 12-8-23-5.jpg

 
Well chit! I forgot to post an update on the jars. They are doing great, I don't see any bad areas on the PCE this time. Let's hope it continues!

Substrate jars 12-8-23.jpg

Substrate jars 12-8-23.jpg

 
Ramblinrose1965 said:
Looking good....might be time for a shake rattle and roll.
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Thanks! Not sure what you mean by shake rattle and roll on the substrate jars? Do you mean time to dunk? 😆
 
Normally with grain jars you shake them up to distribute the mycelium after they reach 40-50% colonization. I'm not sure if you need to shake BRF jars, though.
 
Lady Cannafan said:
Thanks! Not sure what you mean by shake rattle and roll on the substrate jars? Do you mean time to dunk? 😆
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If you shake the jar and break up the colonized grain and redistribute the colonized grain it will colonize the jar much quicker.
 
BeccaBuds said:
Normally with grain jars you shake them up to distribute the mycelium after they reach 40-50% colonization. I'm not sure if you need to shake BRF jars, though.
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My bad .....I thought they were grain spawn.
 
Thanks @BeccaBuds and @Ramblinrose1965
I'm not sure if those are shakeable, but I will try one. I wish I had grain spawn. LOL
 
Welllll, everything is subject to change. 😆
I am going to be moving the Ecosphere 3.0 to a different room. I need the room it's in to veg 5 young plants when I flip the larger plants to 12/12 lighting in the next week.
A growers work is never done......
 
Lady Cannafan said:
Welllll, everything is subject to change. 😆
I am going to be moving the Ecosphere 3.0 to a different room. I need the room it's in to veg 5 young plants when I flip the larger plants to 12/12 lighting in the next week.
A growers work is never done......
Click to expand...
Shrooms, drying shrooms, flipping the girls to 12/12 and nursing clones..... Lady Cannafan you are correct. Its never done lol.
 
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