Vancouver Island 2022 Organic Grow

[Danishoes21]

THE CLONES
This is where we do the full circle and connect the dots. The clones had been sprayed the same and watered the same. One of the Xenomorph phenos is showing her first flat white stain on the leaf. The rest that arent buding show no signs of it. More and more things to consider when looking at this experiment.
They are doing great to the extent of their enviroment. I will let the pictures speak for themself.

 

[Danishoes21]

Frontiers | Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants

Reactive oxygen species (ROS) were initially recognized as toxic by-products of aerobic metabolism. In recent years, it has become apparent that ROS plays an...

www.frontiersin.org

Still reading many other publications.

 

[StoneOtter]

Frontiers | Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants

Reactive oxygen species (ROS) were initially recognized as toxic by-products of aerobic metabolism. In recent years, it has become apparent that ROS plays an...

www.frontiersin.org

Still reading many other publications.

That's over my head.

 

[StoneOtter]

THE CLONES
This is where we do the full circle and connect the dots. The clones had been sprayed the same and watered the same. One of the Xenomorph phenos is showing her first flat white stain on the leaf. The rest that arent buding show no signs of it. More and more things to consider when looking at this experiment.
They are doing great to the extent of their enviroment. I will let the pictures speak for themself.

Nice! They're coming on strong!

 

[Danishoes21]

Antimicrobial Compounds and Cell Wall Metabolism in Infected Maize Leaves​

One of the first defense barriers that pathogens encounter in plants is the production of antimicrobial compounds, such as phenolic compounds related to cell wall metabolism and ROS (Lamb and Dixon, 1997; Heath, 2000). To have a better understanding of the biochemical changes induced in maize plants during biotrophic and necrotrophic development of C. graminicola, we monitored the activation of plant defense mechanisms in a time-course experiment during a compatible interaction. As we wanted to test in vivo responses induced in the plant without the interference caused by leaf detachment (Liu et al., 2007), we only infected leaves on intact plants. Increased levels of phenolic compounds were induced in maize leaves upon infection with C. graminicola. The activation of defense mechanisms was evidenced by the presence of autofluorescence in infected cells 48 hpi (Fig. 2A). This observation was further confirmed by quantitative data showing increased levels of p-hydroxycinnamic acid and phytoalexins 48 hpi (Fig. 2B). The increased metabolism of phenolic compounds and lignin deposition in infected tissue was further demonstrated after toluidine blue staining 72 hpi (Fig. 2C). These results are in agreement with increased levels of mRNA encoding for Phe ammonia lyase, a key enzyme involved in the biosynthesis of p-hydroxycinnamic acid and derivative phenolic compounds acting as building blocks for lignin and several flavonoids, such as phytoalexins and anthocyanins (data not shown).

Figure 2.
Production of antimicrobial compounds associated with plant cell wall metabolism during C. graminicola infection. A, Micrographs showing green autofluorescence in maize cells 48 and 72 hpi after infection. Mock-inoculated plants did not show any green ...




So bare with me on this one, even though this study is made on Maize and is referring specific to blight (a form of fungal disease), the principal remains the same.
I also bold out some key words if any one is interested in understanding.

 

[scottreid1966]

I did some research on Cafe Racer; actually very awesome mix of genes. So you could be seeing a cafe racer that exhibits mostly granddaddy purple pheno traits and could be a GSC chemotype… that’s just me wild guessing. But anyways somewhere down the linage of your current cafe racer are phenotype traits of purple Colors or darker Color.
It’s also saying it could reach 8ft tall outdoors.

Cafe Racer Lineage / Genealogy​

• Cafe Racer »»» Grandaddy Purple x Girl Scout Cookies
• Grandaddy Purple Probably
  • »»» Mendo Purps x {Skunk x Afghanistan} Probably
  • Skunk x Afghanistan
    • Skunk
      • Skunk #1
        • »»» Afghanistan x Mexico x Colombia
        • Afghanistan »»» Indica
        • Mexico »»» Sativa
        • Colombia »»» Sativa
    • Afghanistan »»» Indica
  • Mendo Purps
    • Unknown Mostly Indica »»» Mostly Indica
• Girl Scout Cookies
  • »»» F1 Durban x OG Kush South Florida
  • F1 Durban S1
    • Cherry Pie Kush
      • »»» Durban Poison x ChemdawgMendocino
      • Durban Poison IBL
        • Durban Poison Durban
          • South African SativaKwaZulu-Natal »»» Sativa
      • Chemdawg Mendocino (specified above)
  • OG Kush South Florida Probably
    • »»» Chemdawg x {Lemon Thai x Hindu Kush, Pakistan} Probably
    • Lemon Thai x Hindu Kush, Pakistan
      • Lemon Thai »»» Sativa
      • Hindu Kush, Pakistan »»» Indica
    • Chemdawg
      • Unknown Indica »»» Indica

As an indoor crop, Granddaddy Purple is equally happy in hydro or soil. With a pure indica heritage, this plant is predisposed to a short bushy stature. Granddaddy branches extensively, making her less than ideal for SOG style grows, but she can be trained to make an awesome super crop garden. When left to her natural tendencies, this strain will make a nice big shrub that reaches about 3 feet indoors or up to 8 feet outdoors. She is a hardy grower with tight internodes and dense dark green to purple leaves.

Granddaddy Purple is easy to work with throughout her growth cycle. She likes a cool temperature, between 70-80 F, and can be very forgiving so long as she is adequately watered. Due to her high resin output and dense structure, this plant can be alluring to mites.

Granddaddy Purple finishes her flowering cycle in 8-9 weeks. The buds are dense green nuggets that gain royal purple hues as they mature. Depending on the size she is allowed to reach, Granddaddy Purple can yield between 0.5 and 3.5 ounces apiece. outdoor plants will really deliver, with potential yields between 8 ounces and 5 pounds.

You are awesome, brother!

Planted the outdoors too early. Ergo, the 10 footers. The insides are about 2 and a half to 3. You have no idea how much I appreciate efforts like this. Brings a tear to my eye.

So glad that we met and followed journals.

Have a fantastic Friday, my friend!

I got a good update for yall, like always bare with me its a photo dump.

I will start with my observations; as you know I have been trying many things to fight PM, rust and many other pest and fungal problems that are common for cannabis outdoors, you can't win them all.
After one week of foliar spraying common plantain with PM (anaerobic), and the constant horsetail/neem foliar, I have also used the same liquids as part of my root watering routine, hit them with seaweed, now the fern, banana peel.. like they are getting hit with everything I got roots and foliar.
First thing is I dont see PM all over what I see is consistent stains that are flat white, they are round and seem to be isolated, I dont want to jump into any conclusions, I am leaning with the possibility of thats what it looks like to produce a ROS self defense to PM. I am a believer, I dont doubt those are PM marks on the foliage (the plants are surrounded by PM, it is everywhere) It would be ludacris to think I would be the one with out PM. I like what I see, its new I have never seen a stain like that.
Now I have to do one thing, If i understood the data I have been posting here, some of this ROS responses actually have fluorescent marks or has some sort of bio-luminescence. I yet to see that, I would probably time a visit to see them at night.
Anyways I leave all the photos for you knowledgeable peps to see, digest and discuss.
In this visit I have up the dose (of bio stressor or elicitors Im not sure on what is what), just to say Im using full force of every tea I have both foliar and for roots.
I AM ALL IN>.

CHUNKY SKUNK
Lets start with the one that has the most marks and is showing the most vunerability. She is expressing something on the lower foliage, rapid yellowing and necrosis of lower fans. Is it a flowering phase? I wouldnt know. Is she suffering from the pathogen attack? I dont know. Is she showing more signs of PM fight than the rest because she has some nutrient prolem? could be the case, If she isnt at the top of the health chain she will suffer far more than the rest.
She is also forming buds and thats also a major energy expense. Fight PM and flower equals nutrient deficiency.

Looking good Dani! I thought it was normal for the fan leaves to turn yellow during flower. I sure as heck trimmed a shit-load off of my outside girls today.

Cheers!

 

[scottreid1966]

Antimicrobial Compounds and Cell Wall Metabolism in Infected Maize Leaves​

One of the first defense barriers that pathogens encounter in plants is the production of antimicrobial compounds, such as phenolic compounds related to cell wall metabolism and ROS (Lamb and Dixon, 1997; Heath, 2000). To have a better understanding of the biochemical changes induced in maize plants during biotrophic and necrotrophic development of C. graminicola, we monitored the activation of plant defense mechanisms in a time-course experiment during a compatible interaction. As we wanted to test in vivo responses induced in the plant without the interference caused by leaf detachment (Liu et al., 2007), we only infected leaves on intact plants. Increased levels of phenolic compounds were induced in maize leaves upon infection with C. graminicola. The activation of defense mechanisms was evidenced by the presence of autofluorescence in infected cells 48 hpi (Fig. 2A). This observation was further confirmed by quantitative data showing increased levels of p-hydroxycinnamic acid and phytoalexins 48 hpi (Fig. 2B). The increased metabolism of phenolic compounds and lignin deposition in infected tissue was further demonstrated after toluidine blue staining 72 hpi (Fig. 2C). These results are in agreement with increased levels of mRNA encoding for Phe ammonia lyase, a key enzyme involved in the biosynthesis of p-hydroxycinnamic acid and derivative phenolic compounds acting as building blocks for lignin and several flavonoids, such as phytoalexins and anthocyanins (data not shown).

Figure 2.
Production of antimicrobial compounds associated with plant cell wall metabolism during C. graminicola infection. A, Micrographs showing green autofluorescence in maize cells 48 and 72 hpi after infection. Mock-inoculated plants did not show any green ...




So bare with me on this one, even though this study is made on Maize and is referring specific to blight (a form of fungal disease), the principal remains the same.

Do you have a university degree?
yOU TWO REALLY KNOW YUOUR SHIT! DAM CAPS LOL

 

[Danishoes21]

You are awesome, brother!

Planted the outdoors too early. Ergo, the 10 footers. The insides are about 2 and a half to 3. You have no idea how much I appreciate efforts like this. Brings a tear to my eye.

So glad that we met and followed journals.

Have a fantastic Friday, my friend!



Looking good Dani! I thought it was normal for the fan leaves to turn yellow during flower. I sure as heck trimmed a shit-load off of my outside girls today.

Cheers!

Thanks for your kind words, what can I say I like cannabis too much. People around will say I’m obsessed (so what! Is my answer).

Couldn’t miss out on seeing other people grow massive plants of very cool genetics!
Keep ‘en coming Scott

 

[greenjeans]

Planted the outdoors too early. Ergo, the 10 footers

That's why I waited until June 1 to start this year!!! So far I think I got it OK - they still fit in my laundry tubs and I can fairly easily move them into the greenhouse at night. I'm also hoping they don't seem so tired and old around the middle of October - that's the plan - until I screw up some other way!!!

 

[greenjeans]

That's over my head.

and both sides of mine!!!

 

[Azimuth]

Antimicrobial Compounds and Cell Wall Metabolism in Infected Maize Leaves​

One of the first defense barriers that pathogens encounter in plants is the production of antimicrobial compounds, such as phenolic compounds related to cell wall metabolism and ROS (Lamb and Dixon, 1997; Heath, 2000). To have a better understanding of the biochemical changes induced in maize plants during biotrophic and necrotrophic development of C. graminicola, we monitored the activation of plant defense mechanisms in a time-course experiment during a compatible interaction. As we wanted to test in vivo responses induced in the plant without the interference caused by leaf detachment (Liu et al., 2007), we only infected leaves on intact plants. Increased levels of phenolic compounds were induced in maize leaves upon infection with C. graminicola. The activation of defense mechanisms was evidenced by the presence of autofluorescence in infected cells 48 hpi (Fig. 2A). This observation was further confirmed by quantitative data showing increased levels of p-hydroxycinnamic acid and phytoalexins 48 hpi (Fig. 2B). The increased metabolism of phenolic compounds and lignin deposition in infected tissue was further demonstrated after toluidine blue staining 72 hpi (Fig. 2C). These results are in agreement with increased levels of mRNA encoding for Phe ammonia lyase, a key enzyme involved in the biosynthesis of p-hydroxycinnamic acid and derivative phenolic compounds acting as building blocks for lignin and several flavonoids, such as phytoalexins and anthocyanins (data not shown).

Figure 2.
Production of antimicrobial compounds associated with plant cell wall metabolism during C. graminicola infection. A, Micrographs showing green autofluorescence in maize cells 48 and 72 hpi after infection. Mock-inoculated plants did not show any green ...




So bare with me on this one, even though this study is made on Maize and is referring specific to blight (a form of fungal disease), the principal remains the same.
I also bold out some key words if any one is interested in understanding.

Ok. Thanks for this. I think. But I'm going to need you to explain it to me like I'm 6.

 

[Bill284]

I got a good update for yall, like always bare with me its a photo dump.

I will start with my observations; as you know I have been trying many things to fight PM, rust and many other pest and fungal problems that are common for cannabis outdoors, you can't win them all.
After one week of foliar spraying common plantain with PM (anaerobic), and the constant horsetail/neem foliar, I have also used the same liquids as part of my root watering routine, hit them with seaweed, now the fern, banana peel.. like they are getting hit with everything I got roots and foliar.
First thing is I dont see PM all over what I see is consistent stains that are flat white, they are round and seem to be isolated, I dont want to jump into any conclusions, I am leaning with the possibility of thats what it looks like to produce a ROS self defense to PM. I am a believer, I dont doubt those are PM marks on the foliage (the plants are surrounded by PM, it is everywhere) It would be ludacris to think I would be the one with out PM. I like what I see, its new I have never seen a stain like that.
Now I have to do one thing, If i understood the data I have been posting here, some of this ROS responses actually have fluorescent marks or has some sort of bio-luminescence. I yet to see that, I would probably time a visit to see them at night.
Anyways I leave all the photos for you knowledgeable peps to see, digest and discuss.
In this visit I have up the dose (of bio stressor or elicitors Im not sure on what is what), just to say Im using full force of every tea I have both foliar and for roots.
I AM ALL IN>.

CHUNKY SKUNK
Lets start with the one that has the most marks and is showing the most vunerability. She is expressing something on the lower foliage, rapid yellowing and necrosis of lower fans. Is it a flowering phase? I wouldnt know. Is she suffering from the pathogen attack? I dont know. Is she showing more signs of PM fight than the rest because she has some nutrient prolem? could be the case, If she isnt at the top of the health chain she will suffer far more than the rest.
She is also forming buds and thats also a major energy expense. Fight PM and flower equals nutrient deficiency.

Thanks for the detailed update.
You have quite the battle going, good luck my friend.
Hope you knock it down.
Those spots do give me some concern though.
Hopefully she pulls through.
Keep up the good work.
If you get a minute post a pic in my Heavens Shores thread.
I'd love to have you drop off a pic or two.
Take care.




Stay safe
Bill284

 

[Danishoes21]

Do you have a university degree?
yOU TWO REALLY KNOW YUOUR SHIT! DAM CAPS LOL

Well I do have some degrees, but not in biology or horticulture. I’m just curious, and I like plants. I’m curious of all pest and problems because I grow food too, and many many veggie crops suffer the same mildew, rust, mites, root rot, fruit rot… so it helps me understand how to fight them on every battle ground whether it’s cannabis or cucumber or corn.
I’m always hoping a biologist in the community will one day just start commenting on everyone’s grows lol

 

[Danishoes21]

Thanks for the detailed update.
You have quite the battle going, good luck my friend.
Hope you knock it down.
Those spots do give me some concern though.
Hopefully she pulls through.
Keep up the good work.
If you get a minute post a pic in my Heavens Shores thread.
I'd love to have you drop off a pic or two.
Take care.




Stay safe
Bill284

Thanks for dropping by Bill,

As you can see I am at it, trying to pull my head around it.
The spots look like PM but I’ve seen PM up and close and there seems to be something different here, the hyphae from the PM mycelium is not there or at least I can’t see it without a magnifying glass.

What do you think about the marks?


What I have been applying is all preventive so I am not sure of this is a reaction to it. I’m aware that PM is there also seems only to affect budding plants rather than all plants.
I am waiting for my latest foliar spray to be ready, willow leaf (anaerobic fermented) in hopes of triggering a Salicylic acid effect on the plants, it is natural hormone found in plants and it has many functions one to be antiseptic and anti fungal.

I’ll make sure to to choose a nice photo for the request.

 

[Azimuth]

Maybe it is the intro spot where the pm landed but, because of your spray treatments, it has not progessed?

If so, that would suggest the treatments can prevent, but not reverse, infection.

 

[Danishoes21]

Maybe it is the intro spot where the pm landed but, because of your spray treatments, it has not progessed?

If so, that would suggest the treatments treat, but don't prevent or reverse, affliction.

I’m there with you.

So if that stain is a successful treated affliction, that would mean there shouldn’t be a necrotic second phase?
We shall see.

I’m really looking forward to spray them with the willow extract, I had a look and smell, totally different smell than other organic matter I’ve done, it has a astringent smell (I wouldn’t categorize astringent as a smell but a taste (organoleptic qualities)), it doesn’t smell funky at all, I might even say it smells like medicine lol.

I just burped the jars and the same reaction happens when the oxygen hits the medium, massive effervescent.

 

[Danishoes21]

On a random note,

I’m moving places by the end of the month and started packing shit… this is how much I like herbs and natural medicine;

My stash of goodies, that’s all medicinal, wait till I show you the psychedelic stash lol

I love mushrooms and mycelium, just not on my cannabis.

 

[scottreid1966]

On a random note,

I’m moving places by the end of the month and started packing shit… this is how much I like herbs and natural medicine;

My stash of goodies, that’s all medicinal, wait till I show you the psychedelic stash lol

I love mushrooms and mycelium, just not on my cannabis.

So much to learn from you guys.
Looks like some good medicine there, my friend.

 

[Danishoes21]

So much to learn from you guys.
Looks like some good medicine there, my friend.

Bunch of chaga from Quebec, reishi from here BC, birch polypore also from Quebec… so many different types of medicinal flower… horsetail, rosemary (which is highly anti fungal), thyme, sage… just many many things but for us humans. The plants get some other stuff.

 

[greenjeans]

I’m moving places by the end of the month and started packing shit

Oh Man - I sure don't envy that procedure!!!

Hey Shoes!! My Fern Elixer is about 12 days old now and I'm wondering if 1 teaspoon per gal of water sounds about right?? Do you think it kept the PM at bay?? And how often did you spray??