Patient's Grows
- Thread starter Pat Puffer
- Start date
Paul Squiggle
Well-Known Member
Howdy doo.
I've got some catching up to do. Hope you don't mind me digging up the past and asking questions?
I've got some catching up to do. Hope you don't mind me digging up the past and asking questions?
- Thread starter
- #223
Exhume the posts and ask away. 
squiggy
squiggy- Thread starter
- #224
Well, I was taking off out of town again and a last minute decapitation was executed on the main stalk.
I was thinking of keeping it, then I saw that there were branches that would fill the middle. So...choppy choppy!
I was thinking of keeping it, then I saw that there were branches that would fill the middle. So...choppy choppy!
- Thread starter
- #225
That sad little leaf on the bottom appears to have a Cal deficiency. That leaf rests on the soil at night when she sleeps. I need one of GDB's rocks. 
- Thread starter
- #226
Sad to chop a strong growing shoot...in the idea of an "even canopy", it was crucial.
These high level decisions are daunting some days Patient.
Omg I do almost the same thing. If you add a little bees wax and a few drops of mint, you will have a salve that rocks.Well, I should've known better. I started a season grow journal, though my growing never really stops. I should have just made an all encompassing journal to cover it all. Multiple threads are not as easy to take care of. This thread will chronicle what i do during the winter months when i test strains and make seeds. This will also document me rebuilding my Bonsai section after caterpillars from drying bud decimated my collection. (Sad but true) Rookie mistake and i never said I was a vet.Pillars killed an AC/DC and left me with only 1. So the lone AC/DC goes in to the Bonsai section and i am left with 3 Harleys, one goes in to the Bonsai and 2 go for breeding with my BluSkunk (BluWater×Skunk). So the 1st breeding project is my BluSkunk × Harley Tsu. The BluSkunk has great structure and bud, but it's aroma can be a bit strange. Hopefully outbreeding it will correct the odd bouquet.
Over here we have some seedlings. These are BluLimeSkunk & SourSkunkMango. Odd names but it helps me keep track of the genetic inputs. Further breeding will result and letters and numbers. ROFL! Both have been autos i bred with. Why? Why not. It's what i like to do. These are the 2nd attempt. I had to abort the last attempt at seeds due to taking off out of town and roots weren't developed enough to transplant to bigger pots. While gone the solo cups would have dried up and killed them. Sooo...we plan on another trip and these plants have 1 month to get there for a transplant. I am sure they will make it this time.
Mean while...i am almost out of Coco Oil.Costco offers a larger container than other stores! Awesome.
This is the lecithin i use. 1 Tbsp per 1 Cup of carrier/oil. 54 fl oz = 7 Tbsp. 84 fl oz = 10.5 Tbsp. This is enough to still allow the lecithin to dissolve in the oil. For the 84 fl oz. I used 3.8 oz of dried flower.
I chop by hand to keep from pulverizing and turning to a dust.
I decarb it @240° f for 45 mins. I cover with foil to keep it more covered (optional? ...probably) After that i add to my crock pot and maintain a temp of 150° to 180° for a period of 6 to 12 hours. This method is common. I got my initial info from Corrine Tobias at (wakeandbake) dot something something. She has a great page. She clued me in to lecithin as an emulsifier and an OTC to help battle any negative effects ingesting 95% saturated fat on a daily basis. After 5 yrs of daily ingestion and an okay diet, Kaiser says my cholesterol is great and no signs of high intraocular eye pressure. Pursuant to Jorge Cervantes' method, i allow my oil to sit around for a couple more days before straining. No cooking, just sitting. Then i strain and refrigerate.
*edit
GoogleGenie
Well-Known Member
Man growing in soil is complicated business sometimes. I take my hat off to you guys.
Looking great in here. Ladies are blissfully happy, loving life in your very capable hands @Patient Puffer
Looking great in here. Ladies are blissfully happy, loving life in your very capable hands @Patient Puffer
Very interesting grows my friend. Love your experiments and techniques.Well, I was taking off out of town again and a last minute decapitation was executed on the main stalk.
I was thinking of keeping it, then I saw that there were branches that would fill the middle. So...choppy choppy!
- Thread starter
- #231
4 weeks tomorrow since transplanting the clone.
She has grown better than expected. I have been waiting for all the MotM gifts to show up and thank the sponsors. Still waiting on the Magical Butter Machine.
The Earth Alive soil activator is a keen product I have been researching and it's splendid. I am going to apply next watering following the watering instructions. Optimally it is used before transplant. My thought is "better late than never".
I still plan on flipping her this weekend. I do not have a ton of head room and this AC/DC is a sativa dominant pheno of Catatonic.
She has grown better than expected. I have been waiting for all the MotM gifts to show up and thank the sponsors. Still waiting on the Magical Butter Machine.
The Earth Alive soil activator is a keen product I have been researching and it's splendid. I am going to apply next watering following the watering instructions. Optimally it is used before transplant. My thought is "better late than never".
I still plan on flipping her this weekend. I do not have a ton of head room and this AC/DC is a sativa dominant pheno of Catatonic.
- Thread starter
- #232
...she knows it too.
- Thread starter
- #233
If any one has read this thread, they would have read an article on the use of bacteria to "solubilize the locked up phosphorous" in our soil. The product Earth Alive, aims to do just that.
There are 3 bacteria present in their soil activator.
- Bacillus subtilis
- Bacillus amyloliquefaciens
- Pseudomonas monteilii
...okay, cool. So what does all that mean? I checked some agricultural articles regarding these bacteria...
Bacillus subtilis - Abstract
Plants encounter many biotic agents, such as viruses, bacteria, nematodes, weeds, and arachnids. These entities induce biotic stress in their hosts by disrupting normal metabolism, and as a result, limit plant growth and/or are the cause of plant mortality. Some biotic agents, however, interact symbiotically or synergistically with their host plants. Some microbes can be beneficial to plants and perform the same role as chemical fertilizers and pesticides, acting as a biofertilizer and/or biopesticide. Plant growth promoting rhizobacteria (PGPR) can significantly enhance plant growth and represent a mutually helpful plant-microbe interaction. Bacillus species are a major type of rhizobacteria that can form spores that can survive in the soil for long period of time under harsh environmental conditions. Plant growth is enhanced by PGPR through the induction of systemic resistance, antibiosis, and competitive omission. Thus, the application of microbes can be used to induce systemic resistance in plants against biotic agents and enhance environmental stress tolerance. Bacillus subtilis exhibits both a direct and indirect biocontrol mechanism to suppress disease caused by pathogens. The direct mechanism includes the synthesis of many secondary metabolites, hormones, cell-wall-degrading enzymes, and antioxidants that assist the plant in its defense against pathogen attack. The indirect mechanism includes the stimulation of plant growth and the induction of acquired systemic resistance. Bacillus subtilis can also solubilize soil P, enhance nitrogen fixation, and produce siderophores that promote its growth and suppresses the growth of pathogens. Bacillus subtilis enhances stress tolerance in their plant hosts by inducing the expression of stress-response genes, phytohormones, and stress-related metabolites. The present review discusses the activity of B. subtilis in the rhizosphere, its role as a root colonizer, its biocontrol potential, the associated mechanisms of biocontrol and the ability of B. subtilis to increase crop productivity under conditions of biotic and abiotic stress. (taken from ScienceDirect)
Bacillus amyloliquefaciens - Abstract
This study was aimed to identify plant growth-promoting bacterial isolates from soil samples and to investigate their ability to improve plant growth and salt tolerance by analysing phytohormones production and phosphate solubilisation. Among the four tested bacterial isolates (I-2-1, H-1-4, H-2-3, and H-2-5), H-2-5 was able to enhance the growth of Chinese cabbage, radish, tomato, and mustard plants. The isolated bacterium H-2-5 was identified as Bacillus amyloliquefaciens H-2-5 based on 16S rDNA sequence and phylogenetic analysis. The secretion of gibberellins (GA4, GA8, GA9, GA19, and GA20) from B. amyloliquefaciens H-2-5 and their phosphate solubilisation ability may contribute to enhance plant growth. In addition, the H-2-5-mediated mitigation of short term salt stress was tested on soybean plants that were affected by sodium chloride. Abscisic acid (ABA) produced by the H-2-5 bacterium suppressed the NaCl-induced stress effects in soybean by enhancing plant growth and GA4 content, and by lowering the concentration of ABA, salicylic acid, jasmonic acid, and proline. These results suggest that GAs, ABA production, and the phosphate solubilisation capacity of B. amyloliquefaciens H-2-5 are important stimulators that promote plant growth through their interaction and also to improve plant growth by physiological changes in soybean at saline soil. (taken from NCBI)
Pseudomonas monteilii - Abstract
Rhizosphere provides unique space for intensive chemical conversation between plant and microorganisms. The common rhizobacterial mechanisms which have been demonstrated to promote plant growth include production of phytohormones, nitrogen fixation, synthesis of 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) and phosphate solubilization. The microbially produced phytohormone indole-3-acetic acid (IAA) is considered to have significant role in interaction between plant and bacteria. Hence any substance with modulatory effect on rhizobacterial IAA production can expect to have its impact on plant–microbe interaction. With the advent of nanotechnology, nanoparticles are being used for diverse applications. However, applications of nanotechnology in agriculture have not been studied in detail. In the study, rhizospheric Pseudomonas monteilii was selected to investigate the concentration-dependent effect of biogenic gold nanoparticles (AuNPs) on its IAA production. For this, AuNPs synthesized by Bacillus subtilis SJ15 were characterized by UV–Vis spectroscopy, FT-IR, TEM and EDS. The results showed AuNPs to have spherical, hexagonal and triangular shapes with a size range of 12–32 nm and absorption peak at 545 nm. Further, various concentrations of AuNPs were used to identify its impact on IAA production by P. monteilii. From this, enhanced production of IAA by P. monteilii was found to take place in the presence of 50 µg/mL AuNPs. When Vigna unguiculata seedlings were grown in presence of 50 µg/mL of AuNPs, increased growth was observed. The results of the study thus showed the ability of AuNPs to augment the IAA-producing potential of P. monteilii. (taken from SpringerLink)
...it all sounds pretty far out and almost beyond my comprehension. The results are there. It's not up to me to understand how. I can use this product to enhance my soil's microbial life and my plants will benefit.
There are 3 bacteria present in their soil activator.
- Bacillus subtilis
- Bacillus amyloliquefaciens
- Pseudomonas monteilii
...okay, cool. So what does all that mean? I checked some agricultural articles regarding these bacteria...
Bacillus subtilis - Abstract
Plants encounter many biotic agents, such as viruses, bacteria, nematodes, weeds, and arachnids. These entities induce biotic stress in their hosts by disrupting normal metabolism, and as a result, limit plant growth and/or are the cause of plant mortality. Some biotic agents, however, interact symbiotically or synergistically with their host plants. Some microbes can be beneficial to plants and perform the same role as chemical fertilizers and pesticides, acting as a biofertilizer and/or biopesticide. Plant growth promoting rhizobacteria (PGPR) can significantly enhance plant growth and represent a mutually helpful plant-microbe interaction. Bacillus species are a major type of rhizobacteria that can form spores that can survive in the soil for long period of time under harsh environmental conditions. Plant growth is enhanced by PGPR through the induction of systemic resistance, antibiosis, and competitive omission. Thus, the application of microbes can be used to induce systemic resistance in plants against biotic agents and enhance environmental stress tolerance. Bacillus subtilis exhibits both a direct and indirect biocontrol mechanism to suppress disease caused by pathogens. The direct mechanism includes the synthesis of many secondary metabolites, hormones, cell-wall-degrading enzymes, and antioxidants that assist the plant in its defense against pathogen attack. The indirect mechanism includes the stimulation of plant growth and the induction of acquired systemic resistance. Bacillus subtilis can also solubilize soil P, enhance nitrogen fixation, and produce siderophores that promote its growth and suppresses the growth of pathogens. Bacillus subtilis enhances stress tolerance in their plant hosts by inducing the expression of stress-response genes, phytohormones, and stress-related metabolites. The present review discusses the activity of B. subtilis in the rhizosphere, its role as a root colonizer, its biocontrol potential, the associated mechanisms of biocontrol and the ability of B. subtilis to increase crop productivity under conditions of biotic and abiotic stress. (taken from ScienceDirect)
Bacillus amyloliquefaciens - Abstract
This study was aimed to identify plant growth-promoting bacterial isolates from soil samples and to investigate their ability to improve plant growth and salt tolerance by analysing phytohormones production and phosphate solubilisation. Among the four tested bacterial isolates (I-2-1, H-1-4, H-2-3, and H-2-5), H-2-5 was able to enhance the growth of Chinese cabbage, radish, tomato, and mustard plants. The isolated bacterium H-2-5 was identified as Bacillus amyloliquefaciens H-2-5 based on 16S rDNA sequence and phylogenetic analysis. The secretion of gibberellins (GA4, GA8, GA9, GA19, and GA20) from B. amyloliquefaciens H-2-5 and their phosphate solubilisation ability may contribute to enhance plant growth. In addition, the H-2-5-mediated mitigation of short term salt stress was tested on soybean plants that were affected by sodium chloride. Abscisic acid (ABA) produced by the H-2-5 bacterium suppressed the NaCl-induced stress effects in soybean by enhancing plant growth and GA4 content, and by lowering the concentration of ABA, salicylic acid, jasmonic acid, and proline. These results suggest that GAs, ABA production, and the phosphate solubilisation capacity of B. amyloliquefaciens H-2-5 are important stimulators that promote plant growth through their interaction and also to improve plant growth by physiological changes in soybean at saline soil. (taken from NCBI)
Pseudomonas monteilii - Abstract
Rhizosphere provides unique space for intensive chemical conversation between plant and microorganisms. The common rhizobacterial mechanisms which have been demonstrated to promote plant growth include production of phytohormones, nitrogen fixation, synthesis of 1-aminocyclopropane-1-carboxylate deaminase (ACC deaminase) and phosphate solubilization. The microbially produced phytohormone indole-3-acetic acid (IAA) is considered to have significant role in interaction between plant and bacteria. Hence any substance with modulatory effect on rhizobacterial IAA production can expect to have its impact on plant–microbe interaction. With the advent of nanotechnology, nanoparticles are being used for diverse applications. However, applications of nanotechnology in agriculture have not been studied in detail. In the study, rhizospheric Pseudomonas monteilii was selected to investigate the concentration-dependent effect of biogenic gold nanoparticles (AuNPs) on its IAA production. For this, AuNPs synthesized by Bacillus subtilis SJ15 were characterized by UV–Vis spectroscopy, FT-IR, TEM and EDS. The results showed AuNPs to have spherical, hexagonal and triangular shapes with a size range of 12–32 nm and absorption peak at 545 nm. Further, various concentrations of AuNPs were used to identify its impact on IAA production by P. monteilii. From this, enhanced production of IAA by P. monteilii was found to take place in the presence of 50 µg/mL AuNPs. When Vigna unguiculata seedlings were grown in presence of 50 µg/mL of AuNPs, increased growth was observed. The results of the study thus showed the ability of AuNPs to augment the IAA-producing potential of P. monteilii. (taken from SpringerLink)
...it all sounds pretty far out and almost beyond my comprehension. The results are there. It's not up to me to understand how. I can use this product to enhance my soil's microbial life and my plants will benefit.
Hey PP, that's pretty much over my head too haha, but thanks for posting it. But that's why I like living organic soil because if it contains sufficient phosphorous in rock dusts etc, the living biology will carry on releasing it to the plant roots for years to come. While I am happy with how my soil is currently going, if I was able to purchase 'Recharge' which is available in the States but not here in NZ, I would love to add some of that too, but the product you highlighted, 'Earth Alive', also sounds like a win/win soil amendment.
I did read of someone collecting the root material of his harvested plants and drying it and storing it, until his next grow when he would add some to the root zone of his new plants as a Mycorrhizae additive source. That sounds very promising as the addition of Mycorrhizae is well documented as boosting plant growth, have you heard of this or tried it in your investigations? Cheers.
That sounds like the good stuff Patient! Over my head too but I know sense when I see it. MICROBES HO!
- Thread starter
- #236
@Stunger That is a brilliant idea. I have used Myco, but I no longer apply it. No reason really, I just don't have any.
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- #237
I was about to start my first FPJ (fermented plant juice), but it rained pretty well yesterday. Cho recommends to use plants 2 days after a good rain. The rain washes off the bacteria and will not allow a good ferment to take place.
I already have my little patch of weeds picked out and I will be pulling them tomorrow to start this trip.
I already have my little patch of weeds picked out and I will be pulling them tomorrow to start this trip.
I'm trying some dynomyco in my soil now first time. So far It's too soon to talk about growth and health things. I can say there's a boat load of life growing on top of the soil in the containers vs not in just a few days. I don't get this green growth without it. I'm pretty sure it's brunch for my microbes.
- Thread starter
- #239
I believe that myco is a fungus that aids in the transportation of nutrients; overall postively affecting the plants growth above and below the soil.
That's the hope. What a ton of life in there! Hehe, stoner me forgot to post the pic.