OTM's DIY Bubble Cloning & Sprouting 40 Year Old Seeds & Current RDWC Harvest

DeVille said:
I want to tip you about something that may help you with germination

Germinate valuable and old seeds that need extra care to survive. Kit lets growers clean the seeds' surface of threatening organisms, hydrate the seeds in head start solution, and germinate in sterile media. Kit includes ten tubes of sterilized coco in a tray of tubes, seed washing and hydrating vial with temperature gauge, surface sterilizing compound, hydrating solution, forceps, sealing tape and instructions. Also includes two tissue culture rescue tubes

Microclone Seed Starter Kit
Click to expand...

Thanks for the link and video DeVille, I am looking into this tonight!:thumb:
 
7-19 update;

I did a rez change on both the RDWC gals and the offspring in the bubble cloner this evening...roots are growing fast and so are the momma's, I am still seeing some stretch going on with one of both the Widow & Kush gals in RDWC and the stacking continues.

The only picture tonight is of one of the seeds still soaking, it has split open...going to leave it soaking rather then put it on paper towel to see if a tap starts poking out!

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A great big thanks to DeVille for the link and video above, I plan on doing some research on this method and will probably order up a kit...what is a 50 spot compared to bringing these back!

:thanks:
 
So yesterday I decided to put the cracked beans in the ground in my garden, 5 out of 6 cracked but no taps, setting in a warm paper towel didn't encourage them so its up to Mother Nature now!

I did pull one out of its shell and I can see the start of the tap root, it looks like it has or had the will but maybe not the energy to develop further.:hmmmm:

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I will start 6 more in fresh GA3 solution tomorrow morning upping the dose to 500 PPM, I also ordered the Microclone Seed Starter Kit as suggested by DeVille, it uses a sugar and a mild nutrient/vitamin solution, to hydrate the seeds after soaking them in a H2O2 solution to decontaminate them, something I plan on doing on this round of GA3 after I scuff them.

"If at first you don't succeed, try, try again" :cheesygrinsmiley:
 
7-22 update;

Haven't done a proper update for a few days, it was a busy week outdoors!

The clones are popping and growing nice roots, they are now in a veg solution running 280 PPM's at 5.8 PH, another week of this and they are probably going into big DWC tubs and or Coco Coir...not sure which or it may be a combination of both!

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The current grow is progressing well, stretch is slowing considerably and I am starting to see the bud sites fatten up a bit.

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I am noticing many differences between the strains and with-in the strains, the shorter gals are making nice dense sticky cola's with sugar leaves starting to show.

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The taller ladies have many sites but a lot of spacing between buds, they are not yet getting sticky but are fattening up some, these still are growing tall, not as fast as before, but around a inch every 2-3 days...most are 22-26 inches above the net where the shorter gals are 7-12 inches above. I hope these lanky ladies start to plump up some!:drool:

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I am noticing some leaf discoloration/spotting on one of the Widow gals in RDWC, I noticed this starting as soon as I started adding the Bloom Optimizer the 3rd week of flower, it is not on all areas of the plant and the other Widow in the RDWC shows no signs of this nor does any of the Kush gals.

I called Skunk Labs and explained the problem to them...since this plant was the last to show flower and is behind her sisters in the flower stage, they think it might be a nutrient lock-out on Magnesium and suggested I raise my PH in the system to 6.0 from the 5.6-5.8 I have been normally running it. I was able to get my hand in the bucket to check the roots and no slimy stuff, water flow is not blocked so I upped the PH to 6.0.

I have been running 3 ml per gal on the CaliMagic which is slightly up from the 2.5 ml recommended dose on week 6 and also doing the same with the Coco gals...no problems except for this gal.

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I don't plan on doing anything drastic other than raising the PH as recommended and keeping a close eye on new growth/development.

If you have any suggestions/comments/experience with this problem, let me know your thoughts!:hmmmm:

Tomorrow will be the second try with popping the old beans with GA3 in a higher dose with an additional pre-soak in H2O2, which I didn't do on try #1.

:slide::slide::slide::thanks:
 
My thoughts on the unhappy looking girl is give her a flush and reset the nutrient flow. Otherwise it looks to be at the very least calcium deficiency but usually flushing and re-feeding does the trick if something seems array :thumb: Hoping for your second lot of old beans to pop mate, you got the last batch to crack now just a tap root! :high-five:
 
Night4wings20 said:
My thoughts on the unhappy looking girl is give her a flush and reset the nutrient flow. Otherwise it looks to be at the very least calcium deficiency but usually flushing and re-feeding does the trick if something seems array :thumb: Hoping for your second lot of old beans to pop mate, you got the last batch to crack now just a tap root! :high-five:
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The thing about a closed system and under a scrog is it would mean flush the entire system, and waste what is in the system now, If it doesn't show improvement by the time the next nute change...5 days from today, then that is what is planned unless it starts to show up more or starts to show on the other plants.

What the Skunk Lab guy suggested make sense in a way, this gal, although the same age as her sisters and roommates is the flat chested girl on the last day of school in 8th grade...but when the 9th grade starts...the chest starts to explode! :laughtwo:

I had the start of the tap on the one I popped from its shell, I didn't fool around with the others, maybe they will come alive in dirt, and maybe a bit more GA3 will encourage them...kind of trial and error!:thanks:
 
Not that I don't want to see how the old seeds do, but I'm still pretty keen to see how the flowering is going. We've always been so close in progress. Like you I'm finding the lankiness is an issue. I also took too much in my big defol I think, maybe not on the 24K Golds who had a longer time to recover, but definitely on the LA Confidentials who have the shorter window. You with the scrog had a far different experience there.

For the RDWC/coco decision, did your coco gals hold up with the better growth as things progressed? Or are there other factors in play? I do agree with you that the process of hand feeding in coco for me has ensured I take a close look at everything every day.

As for your deficient plant, I'm noticing the same thing with my LA1. I thought it was the residual effect of the light burn, and I didn't find a pic that matches what I'm seeing in the grow bible. I added Epsom salts a few weeks back and have calcium in my tap water, so I'm not thinking it's a calcium or magnesium deficiency, but what do I know! :laugh:

I have some new seeds coming including some WW freebies, believe it or not I've never had White Widow before. I'll fill you in on my journal eventually!
 
DobeWan said:
Not that I don't want to see how the old seeds do, but I'm still pretty keen to see how the flowering is going. We've always been so close in progress. Like you I'm finding the lankiness is an issue. I also took too much in my big defol I think, maybe not on the 24K Golds who had a longer time to recover, but definitely on the LA Confidentials who have the shorter window. You with the scrog had a far different experience there.

For the RDWC/coco decision, did your coco gals hold up with the better growth as things progressed? Or are there other factors in play? I do agree with you that the process of hand feeding in coco for me has ensured I take a close look at everything every day.

As for your deficient plant, I'm noticing the same thing with my LA1. I thought it was the residual effect of the light burn, and I didn't find a pic that matches what I'm seeing in the grow bible. I added Epsom salts a few weeks back and have calcium in my tap water, so I'm not thinking it's a calcium or magnesium deficiency, but what do I know! :laugh:

I have some new seeds coming including some WW freebies, believe it or not I've never had White Widow before. I'll fill you in on my journal eventually!
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Never hurts to have a few varieties to try!

Both the Widow and Kush Coco gals are small compared to 3 of the gals in the RDWC, my tallest bud site is 10" above the scrog, but all three have well stacked cola's developing, and all three are getting sticky. The other three in RDWC are 10" to 26" above the scrog and I am seeing lots of buds but lots of space between and really no stickiness to the sites.

I think a lot of these differences may be the pheno-type, for example my two short Kush gals in both coco and RDWC have thin skinny leaves growing with the pistils, all are showing sugar and the node spacing is tight. The big one has lots of sites per stem, leaves are skinny but not like the other two, not much in the way of sugar leaves, and just slight sugar showing on the very tops.

The Widow gals similar in a way, both in the RDWC are big compared to sis in the Coco, but sis in Coco has the nice dense sticky buds, the other two no stickiness, and lots of bud with lots of spacing.

If I had to judge right now what is better...the RDWC would win as far as plant height, and growth, when harvest comes, that may change when all is dried and done!:thedoubletake:
 
OTM, I remember all those old strains from the day. I wish I would have been smart enough to squirrel away some nuts to find 40 years later. If there is anything you think I could do to help you get them going let me know. Just a thought of something I would try would be:

1) H2O2 soak
2) GA3 solution ( First round you got them open I would use that PPM)
3) As soon as I seen them opening I would remove them from that, it done its job.
4) I would gently rinse them with distilled water for a few minutes.
5) I would then soak them in some distilled water with some drops of honey dissolved in it ( Maybe 5-6 drops to a cup ) for a couple hours.
6) Then into paper towel soaked with honey water solution so the seeds can get some air.

No proof this would work, but I think it stands a very good chance. Honey has some amazing property's if you look into it.

Good vibes your way! There is no way I can't follow this. When you get some seeds in the future Michigan would be a great place for some to grow :Namaste:
 
Not surprised the RDWC won out in the end - I remember we both thought it odd the coco got an early lead. That said there are worse things you could do than to try 2 clones from the same plant to compare both approaches. You know, for science! :laugh:
 
JustMeds said:
OTM, I remember all those old strains from the day. I wish I would have been smart enough to squirrel away some nuts to find 40 years later. If there is anything you think I could do to help you get them going let me know. Just a thought of something I would try would be:

1) H2O2 soak
2) GA3 solution ( First round you got them open I would use that PPM)
3) As soon as I seen them opening I would remove them from that, it done its job.
4) I would gently rinse them with distilled water for a few minutes.
5) I would then soak them in some distilled water with some drops of honey dissolved in it ( Maybe 5-6 drops to a cup ) for a couple hours.
6) Then into paper towel soaked with honey water solution so the seeds can get some air.

No proof this would work, but I think it stands a very good chance. Honey has some amazing property's if you look into it.

Good vibes your way! There is no way I can't follow this. When you get some seeds in the future Michigan would be a great place for some to grow :Namaste:
Click to expand...

Thanks for stopping by JustMeds! :welcome:

Thank you for the suggestions, I just did the second batch and did do a pre-soak for about an hour in H2O2 after I scuffed them up. I bumped the GA3 to 500 PPM on this round but I do like the idea of distilled water with a little honey and maybe a drop or 2 of Rapid Root once the crack...sounds similar to the Microclone seed starter kit I ordered!

:thanks:
 
Old Bean Cracking Round 2

I am starting to feel real scientific now, this round I took a few more precautions, water temp monitoring, sterilizing all equipment, and treating the beans to a nice H2O2 bath to get rid of any nasty's that are as old as they are! The only thing I didn't do was the surgical gloves and Dr. mask!:laughtwo:

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After I scuffed the in the sandpaper tube, they spent about an hour in a shot glass with about a 90/10% water/peroxide solution, with me stirring them every 15 minutes while I liquefied the GA3.

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I upped the PPM's of the GA3 to 500 and this round I will be using a temp probe (which I sterilized) as a monitor trying to maintain a dark room temp of 78-80 degrees.

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They are now soaking, and I am hoping the second time is a charm!

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I will keep a close eye on them and update as soon as anything starts to develop!

:thankyou:
 
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