Exceptionally High FECO Yields

Cool Beans,
Now to explain how I do not understand ppm and have zero hydro background. I think I should just say dang. I have been wanting @Pennywise or another hydro grower to teach me about it, but embarrassed I can't grasp ppm. Hard time with micro Moles also. Grams and mL are about top of my capabilities at present. Maybe some time on the site Skybound just gave us would help. :)
 
Maritimer said:
Cool Beans,
Now to explain how I do not understand ppm and have zero hydro background. I think I should just say dang. I have been wanting @Pennywise or another hydro grower to teach me about it, but embarrassed I can't grasp ppm. Hard time with micro Moles also. Grams and mL are about top of my capabilities at present. Maybe some time on the site Skybound just gave us would help. :)
Click to expand...


PPM is super simple. I'm certain you know that it means Parts Per Million, and I'm sure you understand the graduation. It's identical to Percent % which is Parts Per Hundred, which immediately makes me think Centa is a prefix (Latin?) for 100. So when you look at a bottle of nutrient, and it has N listed as 1%, I can easily mentally convert that to 10,000 ppm. 1/100 is the same as 10,000/1,000,000.

Once you learn that simple conversion, you can apply it to hydro, food, soil amendments, whatever. If the manufacturer lists the elemental content on the packaging, you can use those numbers to figure out what all is in there.

So when I say 25ppm, of every 1 million parts of water in my foliar spray, 25 of them is triacontanol. That blog entry suggests using anywhere from 0.1 - 1 Tria which is 25-250 times less than 25ppm, hence when my 2nd batch was a lot less than the 25ppm solution.
 
Any questions you might have just ask. Skybound is one of our resident nutrient gurus. Lights, temps, humidity and air movement are all things we can help with.
 
Skybound said:
So when I say 25ppm, of every 1 million parts of water in my foliar spray, 25 of them is triacontanol. That blog entry suggests using anywhere from 0.1 - 1 Tria which is 25-250 times less than 25ppm, hence when my 2nd batch was a lot less than the 25ppm solution.
Click to expand...
I'm almost with you. What amount of water is a million parts add up to?
would it be like 25 ml of 1 liter?
 
SA and GABA Study in progress.​

Willow Extract First Batches notes; Our idea in root form is use free stuff to elicit increased cannabinoid production in flowering cultivars. Research has proven the combination of SA and GABA applied exogenously to cannabis in lab settings and using purchased materials increases the production of cannabinoids. We hobbits want to do it with willow tree leaves and brown rice.
Salicylic acid (SA) is a simple phenolic compound synthesized in a wide range of prokaryotic and eukaryotic organisms, including plants. Leaf and bark of willow tree (Salix sp.) contain large amounts of SA, which was widely used as a medication for pain relief in the ancient world. Both salicylic acid and GABA induced THCAS gene expression.
•There was a significant correlation between THCAS gene expression and THC content.
•The higher amount of THC coincided with the lower amount of CBD.
•Salicylic acid and GABA can be used as elicitor compounds to massively produce THC and CBD.
•Standard protocol based on spraying on plants to elicit specific metabolites which are mainly produced at flowering time and in flowering organs has many benefits alongside other elicitation approaches.
The highest THC content was obtained in 1 mM salicylic acid and 0.1 mM GABA treatments, respectively. The results suggested that salicylic acid and GABA can control the signaling cascades of genes in cannabinoid pathway by changing their expression patterns at critical concentration, and these two compounds can be considered as effective elicitors for commercial cannabinoid production.
GABA
Accumulation of y-Aminobutyric Acid (Gaba) in the Rice Germ during Water Soaking
Studies on the effects of water soaking on the distribution of free amino acids within the kernel of Koshihikari, which is the most popular cultivar of rice in Japan for its high eating quality, indicated that the Gaba content in the germ increased greatly during water soaking. 3) This present report describes the accumulation characteristics of Gaba in the germ of ten rice cultivars with water soaking.The rice grains were then dried to a safe storage level of moisture content of 15% (w/w) and stored in the form of brown rice for 3 to 4 months at room temperature before use. The germ was prepared as described earlier3) by separating it with a 32-mesh screen from the flour obtained by milling the brown rice. Germ samples (0.2 g) were each suspended and incubated in 3.2 ml of deionized water in a test tube (2 x 12.5 cm) at 40°C while shaking (100 strokes/min; 4 cm amplitude). At 0, 1, and 4 h of incubation, 0.8 ml of a 40% (w/v) trichloroacetic acid solution was added to each relevant suspension to terminate the enzymatic activity. Each suspension was filtered through a 0.45-J.tm membrane filter (Advantec Co., Tokyo, Japan), before the filtrate was analyzed for its Gaba content by a Hitachi L-8500 amino acid analyzer as previously reported. 3) In eight of the ten cultivars, the Gaba content in the germ progressively increased as the incubation proceeded. The greatest Gaba accumulation was observed in the germ of Hokkai 269, which reached more than three times the amount contained in Gabaron tea (about 170 mg/lOO g of dry weight) after a 4-h incubation at 40°C. Hokkai 269 is considered to be a very promising source for Gaba because, in addition to the high Gaba productivity in the germ, its germ is very large, amounting to 2.5 to 3 times the percentage weight of germ from the other cultivars (Table). In contrast, Gaba accumulation in the germ of Takanari was negligible. The Gaba content in the germ of Hoshiyutaka, a hybrid line of japonica and indica, increased during the first 1 h of incubation, and then gradually decreased. In the germ of Hokkai 269, simultaneous increases in glutamate and Gaba were observed between the l·h and 4·h incubations, which reflects the proteolytic activity exceeding the glutamate decarboxylase activity.

Note to self= what indica are they talking about?
The germ of the grains stored for 269 days contained a larger amount of Gaba (36.9 mg/IOO g) than did the I 19-day-stored germ, but the Gaba content increased only 4.4 fold to reach 162 mg/l 00 g after 4 h of water soaking. The small increase in Gaba content in the germ during storage suggests that glutamate decarboxylase had little activity in the germ of brown rice with a moisture content of 15% (w/w). The decrease in final Gaba content of the water-soaked germ caused by prolonged storage at room temperature was presumably due to inactivation of glutamate decarboxylase and the proteolytic enzymes. It is thus recommended that the fresh or well-preserved germ be used for Gaba production.
Tbc. :)
calling Japanese grocery store up in orland today. OHIO
 
@Pennywise these folks are talking about what? micro Mole? Is it one hundreth of a ml?
was obtained in 1 mM salicylic acid and 0.1 mM GABA treatments
 
Maritimer said:
I'm almost with you. What amount of water is a million parts add up to?
would it be like 25 ml of 1 liter?
Click to expand...

Any volume. It doesn't matter if we're talking liters or gallons, whatever the volume, we divide it by a million, then count the parts.
 
Maritimer said:
@Pennywise these folks are talking about what? micro Mole? Is it one hundreth of a ml?
was obtained in 1 mM salicylic acid and 0.1 mM GABA treatments
Click to expand...

The mole is defined by whatever element is being considered. Mole is the molecular weight, I think defined by the atomic number in the periodic table of elements. I only have the basics down when talking in moles. I try to steer clear of getting that deep into the science of things. Such precision would need to be known for medical and chemical, but nutrients for plants are much less critical, so less accuracy is needed to achieve a desirable result.
 
Im glad to hear we wont be playing with moles. KISS, keep it simple stupid. :) I think I got the basic idea. Many thanks to both of you for rendering assistance on a Saturday morning no less. The pooch does not understand why we have stopped spending quality time in the woods. Me neither so we are out of here.
 
Lets put this up.
Sitting here looking at my sealed container holding 75 ml of willow concentrate that is 90% pure grain alcohol. I am worried this is far too harsh to throw in a spray bottle and mist cola's. Using Skybound's guidelines Im figuring like this.

5 ml willow concentrate will be added to 1 liter r.o. and could loosely be described as willow concentrate @5 ppm foliar spray.
How does that sound? :bongrip:
 
Straw Hat Stuff;
Today we got busy doing a bit of sanitation work down below the scrog. It is normal and routine cleaning for us to use Lysol anti-septic towelettes all around the runoff collection basins and racking. While down there I spotted a few minor axillary branches that had previously gone undetected. A big hobbit smile came to me.

We are now following Skybounds example and tinkering with some rooting scenarios involving our Willow extract. Just cause we could, we took prepared cuttings and implanted them in a unfertilized batch of medium. Then drawing up 1 ml of concentrate into a syringe we further diluted the willow extract with 2 ml of room temperature ro water. The 3 ml solution in the syringe was shaken for a while to ensure a mixture of contents. Inserting the needle below the medium surface in proximity to the open stems, we injected 1 ml of our mixture. This was repeated with three separate clone cuttings and we will update anything noteworthy. Keep in mind the Willow is also a potential source of ABA, another elicitor we like. :) Besides, playing with monster cropped cultivars in a few weeks may provide many more opportunities to explore branchial elicitation techniques.

Very good Saturday,
Thanks to all
 
Late Straw Hat Bong Hits,
Think about this; Biologists and scientist are providing the following verbiage.

“Several hormones may act in concert, one after another, to regulate a sequence of developmental events. For example, fruit set may be regulated by lAA, fruit growth by GA, fruit ripening by ethylene, and seed maturation and dormancy by ABA.
Because of these interactions among hormones and between hormones and environmental factors, the extent of which we have only recently begun to appreciate, an understanding of plant hormonal response is a complex and difficult fabric to unentangle.” No kiddin

From the above quotes a few things keep catching my eye. The words “may” is used often, making me think they may have something wrong, or out of order. They don’t seem “sure”.

Now what if it goes something like this.
Seed dormancy and maturation gene sequencing may be regulated by ABA. Until environmental cues (temp and moisture content) are detected by the plants, the ABA maintains seed dormancy. Plants produce a physiological response that is dependent on information obtained from the environment as the GRN Genetic Regulatory Network produces a phytohormone called Absisic Acid (ABA). This phytohormone and others have been shown to play a key role in gene pathway signaling throughout the plant, and changes in concentration of the phytohormones can cause a change in development of cannabinoids. :bongrip:
 
Maritimer said:
Lets put this up.
Sitting here looking at my sealed container holding 75 ml of willow concentrate that is 90% pure grain alcohol. I am worried this is far too harsh to throw in a spray bottle and mist cola's. Using Skybound's guidelines Im figuring like this.

5 ml willow concentrate will be added to 1 liter r.o. and could loosely be described as willow concentrate @5 ppm foliar spray.
How does that sound? :bongrip:
Click to expand...

I am able to determine the ppm of most solutions based on the Guaranteed Analysis that actual scientists created using their skills and equipment for testing various parameters. The same is applied to the Triacontanol. I can calculate ppm based on the information that was provided with the product.

Now, if in your research, you are able to track down baseline measurements that say something like X.yy lbs of Willow branches left to steep in pure water for a week will produce XX% percent solution, then you'd have information to measure off of.
 
Maritimer said:
From the above quotes a few things keep catching my eye. The words “may” is used often, making me think they may have something wrong, or out of order. They don’t seem “sure”.
Click to expand...

My experience when studying elemental involvement inside the plant is the same. I "presume" (lol) this is because the collective knowledge in botany is still in its infancy with respect to the existence of man and how recent we began figuring out the science involved. My Hydroponics book when discussing all of the elements commonly used in hydro solutions is sometimes very vague. This just tells me that the author is recognizing that he doesn't yet know as the information is not yet available, such to conclude for certain. When you think about it, every scientific breakthrough we've made is the result of a collection of other scientific breakthroughs made by others in the past.
 
I don't got the gear, just spinning my wheels.
 
Maybe I will get some good info with my power grown package coming Friday. Like Skybound says we need a marker of sorts that has been properly examined and tested.

Until then we will continue to research and play basement cannabis biologist. My Christmas list this year will include beakers and test tubes.

A friend of mine has pointed out how important our 420 family is to me, and that I may have inadvertently offended another member through my use of slang and callous abbreviations. If I offended anyone I whole heart apologize.
Thanks Shed
 
Maritimer said:
Maybe I will get some good info with my power grown package coming Friday. Like Skybound says we need a marker of sorts that has been properly examined and tested.

Until then we will continue to research and play basement cannabis biologist. My Christmas list this year will include beakers and test tubes.

A friend of mine has pointed out how important our 420 family is to me, and that I may have inadvertently offended another member through my use of slang and callous abbreviations. If I offended anyone I whole heart apologize.
Thanks Shed
Click to expand...

FWIW, I've not read this whole journal, but since you and I've been dialoguing, I didn't pick up on any bad vibes.
 
It's all good. I got a bit rough in my dialogue and referred to Japanese researchers in a way not very 420 like. Not here to throw any stones.

I just checked in on our monster crop clones who each received 1 ml of our willow concentrate and 2 ml of water dilution added to syringe. The good news is they are all alive. I say that tongue in cheek not really understanding what the willow extract concentrations are even composed of, much less what percent of the solution.

But if we can get close, who knows :)
 
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