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Nice ....
Thanks Ole Man!!!
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Nice ....
So you're just going to stick the balls out female into the flower room and see where the pollen flies? Probably a good idea with so many strains if the whole goal is seeds. Dusting pollen on one or two lower branches would produce a decent amount of seeds but you'll have shitloads your way. And it'll be much easier. Hopefully you'll get some decent pollen production this time cause those should be some fantastic crosses. Any idea which crosses you'd consider growing out first?
Icemud, you don't get around much anymore, do ya. You need to get out more! You're holed up in here like Rifleman is in his roost. LOL.
Good to see that you're still around. I'll have to catch up on your current journals.
Hey Brotha just Poppin in glad to see your still around
Thanks yea that's the main reason why I want to make seeds is that I am lucky to be in Cali where most of these genetics originate and really happy to be able to get clone only strains. I decided to start making seeds because even thought the chemotypes and phenotypes may not be the exact same as clone only... the genetics are so awesome that they still may produce some amazing phenos down the road.
Also, knowing that the industry is about to go legal, and there is a lot of bio and pharma interest and investment in this industry, I know that eventually genetics will be either legally protected or possibly even GMO in the near future, so why not preserve whats available before the opportunity is gone
Hopefully I get it right this run... I can't believe I nailed the 1st one and the rest have been duds... going to try to replicate the exact directions this time as I did on the first run...
When is it too late to start spraying CS?
What were those directions again? I'm getting ready to do some as well, maybe if we both use the same process that will help sure up some results?
I am thinking about reversing the Panama x Malawi clone whose donor I like the best, and then open pollinate inside my breeding/veg tent and let the PxM pollinate all the other clones, as well as a PGSC x Cherry OG clone. Then I'll have another PGSC x Cherry OG in flower with some other of the PxM, and take clones of the PGSC x Cherry OG to feminize some of it next time around. I have more seeds of that one put away anyway.
I had a seed run fail when I starting spraying when I saw the plant start to flower. I have had success when I start spraying a week or two before they start to flower. This is with autos. I have never tried with a photo.
Although on the 1st successful seed project I didn't document the process all that well, going back to my journal:
Icemud LED Grow Featuring The All New Budmaster COB Technology - White Full Spectrum
It shows that on day 14 of flowering (from the flip) I had balls (male pollen sacks). In my entry of the day, I mentioned that I was spraying only 1x per day, right at lights on, and on a few occasions I did spray the plants 2x per day.
Prior to that entry I had only 1 entry on day 4 of flowering which also confirmed that I was spraying CS only 1x per day, right when the lights came on...
Day 15 flowering I was still spraying, 1x per day.
On Day 21 I mentioned that I did not see pollen yet, just pollen sacks. I also mentioned I stopped spraying about a week ago... so I am guessing that I sprayed probably until around the 16th or so. I do seem to remember spraying them one or 2 times in that last day 15-21 but it wasn't a daily event... By day 16 it seems that was probably the last day of CS spraying according to my entries.
On day 28 of flowering I mentioned that "the other day I brushed the plant and saw pollen fly off of it" so generally if I were to say "the other day" its usually 2 or 3 days ago... not too long... so I think that around day 25 I saw my first hint of pollen.
Icemud LED Grow Featuring The All New Budmaster COB Technology - White Full Spectrum
So it seems to me on that run I only sprayed for 2 weeks and stopped. Only 1x per day when the lights first came on... Other than that I must have had luck on my side.
I just read on another thread here that others were discussing the reversal of plants that came from feminized seeds, vs the plants that came from true male x female breeding and other growers were discussing the issue with getting pollen sacks but no pollen with plants that came from feminized seeds, while they were getting pollen when doing the same procedure to non feminized plants...
Since I only grow from clone I have no way of knowing whether my plants came from feminized seed, or clone. Since I am in Cali, I would estimate that a good portion of my plants came from clone only sources, however strains such as my Ogiesel which is a Cali Connenction strain, may have come from feminized seeds.... my ogiesel produced pollen sacks but no pollen.
I think that may be a factor, but still not sure completely why some produce pollen, some don't.
I think I was spraying too long into the pollen sack production on my last few unsuccessful runs.... but again its hard to say...
Anyhow I am spraying 2 different plants this run with CS, so hopefully 1 will produce.. I will know in a week or 2
Happy New Year, Ice, from your greatest admirer on 420! I plan to read your journal from beginning to end; looks like you got a new very interesting job; I love how you keep evolving....you are the bestest! Here's to a very productive and successful year in the cannabis industry for the both of us in our respective legal and soon to be legal industries and may we both find our place in it to keep being involved and driving them forward.....to the revolution, my friend.....
I have heard anecdotes about people stabilizing strains by continuing self-polinated generations from S1 into S2 and all the way to S8, but you know how people have a tendency to spout off at the mouth without actually having done the research. I do wonder about the possibility of there being a difference when it's a MALExFEMALE plant being self'd versus a SELFxSELF plant that's being self'd. From a scientific standpoint, the process we're really playing with here is meisosis, and I'm not sure that meiosis operates optimally when performing its tasks with the same DNA. I have a feeling that there may be a sort of "breakdown" in the meisosis process when dealing with the same DNA, and much like with inbreeding, that there could be "coding" errors where offspring's do not get the entire chromosome. This produces deformities which are usually pretty apparent in my experience running up to S2 generations; you'll see a lot of things like webbed leaves for example. But I do wonder if such deformities can express in other things, such as producing staminate flowers that don't produce pollen. I don't think you're going to find any literature that will shed light on this, as I've been scouring academic databases trying to find studies regarding sex determinism in cannabis, and I haven't found anything so in depth. The primary research in this particular field is squarely on our shoulders.
So, just to clarify, are you finding that in your first experiment you stopped spraying silver earlier than in your latter (unsuccessful) experiments? So far the person I've found who has had the most success and who has spoken with experts in the field, is electro gypsy, and he has suggested that less treatments are better, but that at least ONE treatment after pollen sacks are visible may be needed to really fully achieve the effect we're after.
I really wish I could find a source for this... But what I've heard is that this whole process blocks ethylene by introducing silver, which apparently binds with ethylene, and blocks this hormone from acting in the plant as it normally would. Spraying in the morning may actually have been the biggest factor here, as in the morning your stomata are going to be wide open, and will have the must unimpeded absorption of silver particles. There's certainly a lot of variables to consider.
Personally, I don't really see a lot of reason why seeds from a self'd (i.e. feminized) seed source wouldn't have the same effect as one from a "regular" (male x female) source. Of course there's always the possibility there's mechanisms beyond our understanding at play, but simply from the standpoint of meiosis, I don't see a reason why this would come into play unless the feminized sources was actually well-along in terms of successive generations. In other words, if a strain comes out and is an S4 seed stock, then it has been feminized and self pollinated against itself 4 times, and I could see at THAT point the possibility that the ethylene-blocking process may be degrading at that point. However, that is simply based on observing other deformities and abnormalities that occur while the successive self-pollination proceeds, and these may not be directly reflective of the production of pollen.
Lots and lots of variables to consider here. I think your best best ( as you've obviously concluded ) is to mirror the process that netted you your successful attempt. *crosses fingers*
Apologies if I rambled, I'm a little high and drunk. 40 ounces and joints for the win.