Brassinosteroids
What do we know about the brass and can it help us with gardening cannabis?
As they say, this is “yet to be fully elucidated” and what that means is we know a bit, but it changes with each new discovery, but yes brass can and does. Brassinosteroids are are a class of polyhydroxylated steroidal plant hormones that are involved in basically every aspect of plant growth and development. I use red or green cabbage or brown rice from the market, and Willow and American Sycamore tree leaves I collect each spring and fall when brewing up some brass. Usually these home brews are administered to a few of my houseplants prior to getting even close to the cannabis. Sort of like making a younger and smaller sibling taste or eat something before you are willing to try it on yourself, kind of thing. We last used a willow brass in effort to germinate old seeds. The amount of fun I get running these studies makes them worth my effort even in the face of repeated frustration over them mostly not working or worse. We never gonna know if we don't try, I hear.
Some good studies relevant to our efforts with cannabis;
Nie/Huang/Wang/Mao 05/2019
Brassinosteroids (BRs) regulate plant growth and stress responses. BRASSINOSTEROID-INSENSITIVE 1 (BRI1) is a BR receptor that perceives BRs and subsequently activates BR signaling. However, how BR contents and BRI1 expression levels affect the drought resistance of tomato requires further investigation. Here, we exogenously applied 24-epibrassinolide (EBR) and brassinazole (Brz) to tomato plants and generated different transgenic tomato SlBRI1 overexpression lines to study the drought stress response. Our results showed that EBR application 3 days before drought stress increased the contents of BRs and decreased abscisic acid (ABA) and reactive oxygen species (ROS), after which stomatal aperture and drought resistance eventually increased. Brz application reduced the drought resistance. Astonishingly, overexpression of 35S:SlBRI1, which increased BR signaling intensity, led to slightly improved contents of ABA and ROS and ultimately reduced both stomatal aperture and drought resistance. Moreover, plants expressing SlBRI1 driven by a stress-inducible promoter (Atrd29A) also exhibited reduced plant drought resistance. In all cases, enhancing the BR signaling intensity reduced antioxidant enzyme activity and reduced the expression of drought stress-related genes, ultimately compromising the drought resistance. Additionally, SlBRI1 mutants with altered brassinolide sensitivity (abs), which was weak BR signaling, exhibited significantly increased drought resistance. Therefore, our results reveal that BR contents positively regulated tomato drought resistance and that BR signaling intensity via BRI1 was negatively related to the drought resistance. These imply that the increased drought resistance in response to BRs is a newly discovered BR signaling branch that is located downstream of BRs and that differs from that of BRI1.
Barket/ 11/2017
Brassinosteroids (BS) are a class of polyhydroxylated steroidal plant hormones. They are of ubiquitous occurrence in plant kingdom and are implicated in a wide range of physiological, biochemical and molecular responses in plants, such as seed germination, cell division and elongation, vascular differentiation, photomorphogenesis, photosynthesis, enzyme activation and senescence. They have also been found to protect plants from various abiotic and biotic stress factors, such as salt, temperature, water, heavy metals and pathogens. BS also enhance the yield of several cereal, legume, and oil seed crops. Moreover, they also increase the fruit production and quality of the fruits, in different plants of horticultural importance.
Ha/Shang/Yang/Nam 09/2018
Proper regulation of stomatal movement in response to various environmental stresses or developmental status is critical for the adaptation of many plant species to land. In plants, abscisic acid (ABA)-induced stomatal closure is a well-adapted method of regulating water status. In addition to ABA, we previously showed that plant-specific steroidal hormone, brassinosteroid (BR), also induces stomatal closure; however, BR modulates ABA-induced stomatal closure negatively at high concentrations. In this study, we further investigated the cross-talk between ABA and BR in relation to stomatal movement. In contrast to previous reports that ABA-induced stomatal closure was inhibited by brassinolide (BL), the most active BR, we showed that BL-induced stomatal closure was enhanced by ABA, indicating that the sequence of ABA or BL treatments led to different results. We found that this phenomenon occurred because the guard cells still had the capacity to be closed further by ABA, as the degree of stomatal closure by BL was always less than that by ABA. We also found that BL-induced stomatal closure required Open Stomata 1 (OST1) activity and the induced expression of OST1 was indifferent to the sequence of ABA and/or BL treatments. In addition, we examined the underlying mechanism by which inhibition of ABA-induced stomatal closure by BL occurred. We revealed that the downregulation of ABA-biosynthetic genes by BL resulted in a lower accumulation of ABA. These results suggested that the regulation of stomatal movement is finely controlled by the combined effects of plant hormones, ABA and BR.
Pandey/Devi/Singh 07/2020
Brassinosteroids (BRs) are well-characterized growth hormones that are critical for plant growth, development, and productivity. Genetic and molecular studies have revealed the key components of BR biosynthesis and signaling pathways. Insights from Arabidopsis research revealed tissue and cell type-specific roles of BR in controlling cell elongation and maintenance of stem cell niche in roots. More recently, BRs have gained much attention in regulating the root growth during nutrient deficiency such as nitrogen, phosphorus, and boron. Differential distribution of nutrients in the rhizosphere alters BR hormone levels and signaling to reprogram spatial distribution of root system architecture (RSA) such as a change in primary root growth, lateral root numbers, length, and angle, root hair formation and elongation. These morpho-physiological changes in RSA are also known as an adaptive root trait or foraging response of the plant. In this review, we highlight the role of BRs in regulating RSA to increase root foraging response during fluctuating nutrient availability.
to be continued...