Jacob Bell
New Member
Supported by contract Nos. 271-80-3705, 271-80-3721, and 271-81-3802, National Institute on Drug Abuse.
Mario Perez-Reyes MD, Stephanie Di Guiseppi BS, Andrew P Mason BS and Kenneth H Davis BS Chapel Hill and Research Triangle Park, N.C.
Department of Psychiatry and Division of Medicinal Chemistry, The University of North Carolina at Chapel Hill, and Research Triangle Institute
Correspondence: Mario Perez-Reyes MD, Medical Research Building A, Department of Psychiatry, University of North Carolina School of Medicine, Chapel Hill, NC 27514.
Received 25 September 1982; Accepted 14 December 1982.
Top of page
Abstract
Three studies in which exposure to marihuana smoke was progressively increased were conducted. In each, four experienced marihuana users smoked marihuana cigarettes in the presence of two nonsmoking subjects. They were confined in a closed environment for 1 hr after the smoking began. All urine voided by the nonsmoking subjects was collected separately for 24 hr and analyzed for the presence of cannabinoids by the enzyme multiplied radioimmunoassay technique (EMIT) cannabinoid assay. Blood samples were drawn from one of the nonsmoking subjects in one of the studies, and Delta9-tetrahydrocannabinol (THC) plasma concentrations were determined by radioimmunoassay. The results obtained indicate that of 80 urine samples collected in the three studies, the drug levels in only two slightly exceeded the value of 20 ng/ml on the calibrator of the EMIT assay. Minute but detectable concentrations were present in the plasma of the one subject from whom blood samples were obtained. In this subject intravenous infusion of THC at the rate of 3.2 mugl/min for 1 hr resulted in THC plasma concentrations identical to those during his passive inhalation of marihuana smoke.
Source: Abstract of article: Passive inhalation of marihuana smoke and urinary excretion of cannabinoids
Mario Perez-Reyes MD, Stephanie Di Guiseppi BS, Andrew P Mason BS and Kenneth H Davis BS Chapel Hill and Research Triangle Park, N.C.
Department of Psychiatry and Division of Medicinal Chemistry, The University of North Carolina at Chapel Hill, and Research Triangle Institute
Correspondence: Mario Perez-Reyes MD, Medical Research Building A, Department of Psychiatry, University of North Carolina School of Medicine, Chapel Hill, NC 27514.
Received 25 September 1982; Accepted 14 December 1982.
Top of page
Abstract
Three studies in which exposure to marihuana smoke was progressively increased were conducted. In each, four experienced marihuana users smoked marihuana cigarettes in the presence of two nonsmoking subjects. They were confined in a closed environment for 1 hr after the smoking began. All urine voided by the nonsmoking subjects was collected separately for 24 hr and analyzed for the presence of cannabinoids by the enzyme multiplied radioimmunoassay technique (EMIT) cannabinoid assay. Blood samples were drawn from one of the nonsmoking subjects in one of the studies, and Delta9-tetrahydrocannabinol (THC) plasma concentrations were determined by radioimmunoassay. The results obtained indicate that of 80 urine samples collected in the three studies, the drug levels in only two slightly exceeded the value of 20 ng/ml on the calibrator of the EMIT assay. Minute but detectable concentrations were present in the plasma of the one subject from whom blood samples were obtained. In this subject intravenous infusion of THC at the rate of 3.2 mugl/min for 1 hr resulted in THC plasma concentrations identical to those during his passive inhalation of marihuana smoke.
Source: Abstract of article: Passive inhalation of marihuana smoke and urinary excretion of cannabinoids
