I've been following
@Gee64's work for a while now and it definitely does matter where you take the leaves from. He will explain. Also, he does not defol. I'm sure Gee won't mind me commenting here while he is in the land of nod
In a perfect plant all readings should theoretically be the same, but I haven't found a perfect plant yet.
The lower down you go on the plant, the lower the readings generally are.
I use the newest leaves that are completely full size, so about 3/4 the way up the plant. Lots of things can give you a low brix reading.
If you are using synthetics you will find it really hard to get your brix over 12.
Five things mainly control brix, or at least have the biggest effect on it. Calcium, phosphorus, oxygen, carbon, and enough healthy microbes. If you get those 5 dialed brix will climb. And you need adequate light of course.
Too much nitrogen will crash brix so keep that in mind. Nitrogen is aminos, which are precursers to protein. Processing nitrogen into proteins requires a lot of water. All the extra water literally waters your sap down and brix levels drop.
If your brix levels are higher down bottom than up top you are low on phosphorus.
If the line in the refractometer (sorry digital guys, analog is better here) you are low on calcium. If its really fuzzy you are good on calcium.
When you read the leaves, just use the leaves, not the leaf petiole, it will be substantially lower.
Taking readings from OTHER than the leaf won't really tell you much, as the leaf is where things get stored. How much you have stored in your leaves determines whether or not pests want to bite those leaves.
From google
"Grasshopper group – long-horned grasshoppers (Tettigoniidae), crickets, and related insects. They will target a plant when the Brix falls below 12 but will lose interest in it when the Brix sinks below 10.
Chewing insects – beetles (Coleoptera) and butterflies and moths (Lepidoptera) and related insects. They lose interest in a plant when the Brix value reaches as high as 9 to 11. Below that range, a plant is vulnerable to chewing insects.
Sucking insects – leafhoppers (Cicadellidae), froghoppers (Cercopoidea), planthoppers (Fulgoromorpha), stink bugs (Pentatomidae) and thrips (Thysanoptera). They begin to lose interest in a plant when the Brix value reaches between 7 and 9.
Aphid group – aphids (Aphidoidea) and scale (Coccoidea). The insects in this group prefer a really low Brix value, below 6 to 8, because they can’t tolerate too much sugar at once."