If I may just jump in here... the bud washing works so well... I will wash every harvest. I have pugs!! Buds are sticky!! Do the math!! LOL
I'd just say that one can do the wash with confidence if staying with certain parameters.
1. Water cannot be either too hot or too cold. Trichomes are oil based and while not washed off, cold can make them brittle and fall off, and hot can soften them making them more susceptible to being ruptured. My first wash, the hot bath was 82 and the cold rinse 52 and I did see more trichs at bottom of bucket and on sides than I'd have liked, with a smattering of headless pedestals to attest to the loss. Second time was 78 for warm bath and 60 for cold rinse and saw very few trichs lost. That said, My oil yields were good with both, but why loose more than you have to.
I'd be interested in others observations with temps they are using in washes. We should dial that in so we can steer others in the right direction.
2. Duration and agitation... they don't need to be dunked for long unless plagued by white powdery mildew or pests. A 30 second dunk is fine. They also don't need a great deal of agitation. Just moving them slowly back and forth with a couple up and down dunks and they're good to go. I skimmed hair and stuff off top of wash buckets throughout the process to not re-contaminate. Under a scope, I really had to look to find anything on the buds but juicy trichomes!
I think that's it. They dried much faster after the wash than the air dried sample cola I took earlier to test it out before harvest. Makes sense because I tested a water cure on a few colas and it dried two weeks faster than those I just washed. Both were then dried low and slow in fridge. The water cure was curing in jars before the other was even dry.
BTW, I did the water cure for five days. It took most the aroma out of them into the water. So terpenes don't survive the soak very well, but I have to say it's come back close to the other as it's cured. Also the buds shrunk into super compact little nuggets. Lost all bag appeal( which I don't care about anyway). But I will use the water cure again if I need to take extreme measures to save a harvest from the compost pile due to powdery mildew or bud rot. These three colas each had one bud with bud rot. Those are the ones I water cured. Simply cut away bud till no dark core of stem was present, submerged them in RO water with a dash of H202 which was changed once a day, and they turned out perfect.