re: Cardboard Flowerbox Test 3 - 41 Week Perpetual SOG
I think I need to know your clone setup and cloning 101
I'm having issues at the moment and I'm not really sure what that issue is. I may PM you tomorrow about it
It's late and I have to get to bed
Cloning: Mad Simple
Cloning: Mad simple
by
@jandre2k3, 01-06-2012 at 02:02 PM (72 Views)
WHAT YOU'LL NEED
RUBBING ALCOHOL
SURGICAL, OR EXAMINATION GLOVES (unless you're a hand washer)
SMALL SNIPS OR SCISSORS - Must be sharp!!
A SINGLE EDGE RAZOR BLADE - Must be sharp!! A new blade is best.
BACK-UP BLOCK - A piece of wood or plastic or cutting board
SHOT GLASS -Or similar small glass or plastic container.
SPRAY BOTTLE WITH WATER
SEED TRAY WITH CLEAR HUMIDITY DOME
GROWING MEDIUM - One of any of several growing media available at your local hydroponics stores (NOT SOIL).
ROOTING HORMONE - You will need a good quality rooting hormone. You can use a powdered rooting hormone but in My opinion they are much harder to work with resulting in a reduced success rate. Alternatively, you can make a "tea" of willow branches.
BEFORE YOU START
It is critical that you sterilize everything before you start, because cuttings are very susceptible to fungus, viruses and diseases until they root. Everything, even YOU must be clean, Use rubbing alcohol on your gloves (or sanitizer on your hands), the snips, the razor blade and the cutting block. Rinse the shot glass (or whatever you are using) with alcohol, dry it and then fill it 3/4 full with rooting hormone, and set it aside for now.
This might sound obvious, but you should only use very healthy cannabis plants to take cuttings from. The healthier the "mother" cannabis plant(s) the better success you will have with your clones. You should take a few more cuttings than you need and then select the best ones to use and throw away any that are unhealthy.
TIP: You can leach the nitrogen out of the mother cannabis plant(s) by watering them heavily with pH adjusted water only (no fertilizer) for two or three days before you take the cuttings, because the nitrogen stored in the cannabis plant can retard rooting.
Decide what you are going to use for growing medium. Most people use either Rockwool, Rapid Rooters, or Oasis cubes, which are probably the easiest to use because they are pre-formed and already have a hole in them for the cutting. However, you can use many different mediums just as successfully. For instance, you could use plastic cups with holes cut in the bottom to hold coconut fiber, a Perlite & Vermiculite mix or other loose type growing medium. Do not use regular dirt, potting soil, or Peat Pellets as they stay too wet and will rot the stem. These media can also harbor a myriad of diseases and parasites, Depending on where you get them, regular dirt especially. Not to mention the tiny little particulate matter in soils could "clog" the cut end of the stem, thus blocking nutrient uptake and causing health problems later. You will need to pre-soak your growing medium before you start, using pH balanced water. Distilled water is the best thing to use, but any good water source will work. NOTE: Most growing media need to be soaked in pH 5 to pH 7 water, (Rockwool needs to be treated with water adjusted to a pH of 5.5 then rinse-to-waste with some of your nutrient solution as it contains lime from the manufacturing process).
Make holes in the top of the growing medium about the same size or a little smaller than the stems of the cuttings. You do not want to force the cutting into the growing medium.
Work quickly but carefully. When you make the cut that separates the clone from the mother, you must get it into the rooting hormone as quickly as possible to prevent air from getting pulled into the stem.
TAKE THE CUTTINGS
Using the snips, take a growing tip from your cannabis plant 3" to 6" long with at least one leaf internode, two is better but not always possible. (A leaf internode is where the leaf connects to the stem) cut approximately 1/4" below the internode(s)
With the snips, carefully cut off one or two leaves (or small branches) at the cut end of your clone flush with the stem.
With the razor blade slightly below where you just trimmed the leaves. Cut at a 45 to 60 deg. angle. Make this cut as quick and clean as you can, you do not want to tear or crush the stem.
Immediately insert the cutting into the rooting hormone. If you are using a liquid hormone you can let the cutting soak for 30-60 seconds. (NOTE: With a gel type hormone you just dip the cutting and then insert it into the growing medium).
Insert the cutting into the growing medium, do not push the cutting all the way thru the medium, leave room for the roots to develop. Make sure that the cut(s) you made at the leaf internode(s) are below the surface of the growing medium.
Gently pack the growing medium up against the cutting. Make sure there is good contact between the cutting and the growing medium.
CARE AND FEEDING INSTRUCTIONS
Congratulations! You have now successfully taken cuttings or clones, now the challenge is to keep them alive long enough to root. The cuttings are obviously very vulnerable at this point and must be handled with care. If you follow the steps below you should have few problems.
Once you have your cutting(s) in the growing medium mist them with the spray bottle filled with water and place them in the tray. Mist the inside of the clear humidity dome and place dome over the tray. (NOTE: The cuttings need some ventilation and a couple of small holes in the dome will usually do the trick).
For the first 5-7 days, remove the dome and mist the cuttings 2 or 3 times a day. This step is critical as the cuttings have no way to replenish lost moisture because they have no roots. Mist the inside of the dome and replace on tray. This keeps the cuttings from drying out and also humidifies the air inside the dome. The humidity under the dome needs to be about 90 % until roots appear on the cuttings. (NOTE: Be careful that the air under the dome doesn't get too warm, if it is, you may have to increase the amount of venting, and increase the number or mistings per day). Cuttings need to be kept between 72 and 80 degrees Fahrenheit as temperatures too hot or too cold will inhibit root growth. If you live in a cold climate you may need a heated propagation mat.
After about a week you can test to see if your cannabis plants have started to root. Remove the humidity dome and leave it off for an hour or two. If the cannabis plants have not wilted at all then they probably have enough root development to support themselves. If no wilt is noticed leave the dome off, if they are wilted, spray the cuttings and dome and replace the dome on the tray. Once you have determined that the cannabis plants can support themselves, stop misting the cuttings and leave the humidity dome off. (NOTE: Once the cannabis plants have roots, constant misting can actually be harmful to the cannabis plants).
To water the cuttings you can use either plain distilled water (or other Good Quality Water), or you can add a very mild fertilizer at 1/4 strength for most general purpose hydroponic fertilizer solutions. Don't forget to adjust the pH of the water/nutrient solution (5.5 for hydroponic media, 6.5 for most everything else).
Water the cuttings every 2 days unless you live in a very dry climate then you should water every day. Just make sure to NEVER let the growing medium dry out. Also, never let your cuttings sit in water, or the stem will rot. (NOTE: A great way to water the cuttings is to use two trays, one with holes and the other without. Nest the trays together with holes inside, and pour in your water or weak nutrient solution. Let them sit for a few moments and then slowly raise the tray with holes out of the other and let it drain). Small Hydroponic systems are available to automate the watering cycles of the cuttings, greatly reducing the amount of manual labor.
If the lower leaves start to turn yellow and die, don't worry, it is perfectly normal. It is the cannabis plant feeding off of itself to sustain life, moving valuable nutrient and water from the older growth. Do not remove any dead growth until the cannabis plant is well rooted. If you remove the dying growth the cannabis plant can starve and die completely.
Water isn’t the only thing that makes plants grow, the cuttings are going to need light, you need bright light, but not too intense. If you are leaving your cuttings outside, look for a place with dappled sunlight. Indoors you can use a double tube fluorescent fixture with both a cool white bulb and a warm white bulb in the fixture (or two "grow" tubes). This will give a good, balanced light spectrum. Fluorescent light doesn't penetrate so you must keep the lights very close to the cannabis plants (1-2 inches above the cannabis plants).
It is not highly recommended, but, If you are using a Metal Halide or High Pressure Sodium fixture you must keep the cuttings much further away (2 - 3 feet away for 175 - 400 watt bulbs and 4 - 6 feet for a 1000 watt bulb).
In either case the lights should be turned on for 18 to 24 hours a day. I run 24/7 T5 HO until I see roots, then one more week afterward, then I use Twelve12's lighting method, Gas Lantern Routine (12/1):
12 hours on.
5.5 hours off.
1 hour on.
5.5 hours off.
When the cuttings are completely rooted you can move them into your hydroponic system or the soil.
