Jacob Bell
New Member
Cannabidiol decreases bone resorption by inhibiting RANK/RANKL expression and pro-inflammatory cytokines during experimental periodontitis in rats
Author(s) Napimoga MH, Benatti BB, Lima FO, Alves PM, Campos AC, Pena-Dos-Santos DR, Severino FP, Cunha FQ, Guimarães FS
Institution Laboratory of Molecular Biology, University of Uberaba, Brazil. marcelo.napimoga@uniube.br
Source Int Immunopharmacol 2009 Feb; 9(2):216-22.
MeSH Animals
Anti-Inflammatory Agents, Non-Steroidal
Bone Resorption
Cannabidiol
Cell Movement
Cytokines
Disease Models, Animal
Male
Neutrophils
Periodontitis
Peroxidase
RANK Ligand
Rats
Rats, Wistar
Receptor Activator of Nuclear Factor-kappa B
Abstract Cannabidiol (CBD) is a cannabinoid component from Cannabis sativa that does not induce psychotomimetic effects and possess anti-inflammatory properties. In the present study we tested the effects of CBD in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying these effects. Periodontal disease was induced by a ligature placed around the mandible first molars of each animal. Male Wistar rats were divided into 3 groups: control animals; ligature-induced animals treated with vehicle and ligature-induced animals treated with CBD (5 mg/kg, daily). Thirty days after the induction of periodontal disease the animals were sacrificed and mandibles and gingival tissues removed for further analysis. Morphometrical analysis of alveolar bone loss demonstrated that CBD-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-kappaB ligand RANKL/RANK. Moreover, gingival tissues from the CBD-treated group showed decreased neutrophil migration (MPO assay) associated with lower interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha production. These results indicate that CBD may be useful to control bone resorption during progression of experimental periodontitis in rats.
Language eng
Pub Type(s) Journal Article
Research Support, Non-U.S. Gov't
PubMed ID 19070683
Source: Cannabidiol decreases bone resorption by inhibiting RANK/RANKL expression and pro-inflammatory cytokines during experimental periodontitis in rats
Author(s) Napimoga MH, Benatti BB, Lima FO, Alves PM, Campos AC, Pena-Dos-Santos DR, Severino FP, Cunha FQ, Guimarães FS
Institution Laboratory of Molecular Biology, University of Uberaba, Brazil. marcelo.napimoga@uniube.br
Source Int Immunopharmacol 2009 Feb; 9(2):216-22.
MeSH Animals
Anti-Inflammatory Agents, Non-Steroidal
Bone Resorption
Cannabidiol
Cell Movement
Cytokines
Disease Models, Animal
Male
Neutrophils
Periodontitis
Peroxidase
RANK Ligand
Rats
Rats, Wistar
Receptor Activator of Nuclear Factor-kappa B
Abstract Cannabidiol (CBD) is a cannabinoid component from Cannabis sativa that does not induce psychotomimetic effects and possess anti-inflammatory properties. In the present study we tested the effects of CBD in a periodontitis experimental model in rats. We also investigated possible mechanisms underlying these effects. Periodontal disease was induced by a ligature placed around the mandible first molars of each animal. Male Wistar rats were divided into 3 groups: control animals; ligature-induced animals treated with vehicle and ligature-induced animals treated with CBD (5 mg/kg, daily). Thirty days after the induction of periodontal disease the animals were sacrificed and mandibles and gingival tissues removed for further analysis. Morphometrical analysis of alveolar bone loss demonstrated that CBD-treated animals presented a decreased alveolar bone loss and a lower expression of the activator of nuclear factor-kappaB ligand RANKL/RANK. Moreover, gingival tissues from the CBD-treated group showed decreased neutrophil migration (MPO assay) associated with lower interleukin (IL)-1beta and tumor necrosis factor (TNF)-alpha production. These results indicate that CBD may be useful to control bone resorption during progression of experimental periodontitis in rats.
Language eng
Pub Type(s) Journal Article
Research Support, Non-U.S. Gov't
PubMed ID 19070683
Source: Cannabidiol decreases bone resorption by inhibiting RANK/RANKL expression and pro-inflammatory cytokines during experimental periodontitis in rats
