Jacob Bell
New Member
A qualitative and quantitative HPTLC densitometry method for the analysis of cannabinoids in Cannabis sativa L
Author(s) Fischedick JT, Glas R, Hazekamp A, Verpoorte R
Institution Division of Pharmacognosy, Institute of Biology, Leiden University, Leiden, The Netherlands. jtfische@gmail.com
Source Phytochem Anal 2009 Sep-Oct; 20(5):421-6.
MeSH Cannabinoids
Cannabis
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Densitometry
Flowers
Linear Models
Molecular Structure
Reproducibility of Results
Species Specificity
Tetrahydrocannabinol
Abstract INTRODUCTION: Cannabis and cannabinoid based medicines are currently under serious investigation for legitimate development as medicinal agents, necessitating new low-cost, high-throughput analytical methods for quality control.
OBJECTIVE: The goal of this study was to develop and validate, according to ICH guidelines, a simple rapid HPTLC method for the quantification of Delta(9)-tetrahydrocannabinol (Delta(9)-THC) and qualitative analysis of other main neutral cannabinoids found in cannabis.
METHODOLOGY: The method was developed and validated with the use of pure cannabinoid reference standards and two medicinal cannabis cultivars. Accuracy was determined by comparing results obtained from the HTPLC method with those obtained from a validated HPLC method.
RESULTS: Delta(9)-THC gives linear calibration curves in the range of 50-500 ng at 206 nm with a linear regression of y = 11.858x + 125.99 and r(2) = 0.9968.
CONCLUSION: Results have shown that the HPTLC method is reproducible and accurate for the quantification of Delta(9)-THC in cannabis. The method is also useful for the qualitative screening of the main neutral cannabinoids found in cannabis cultivars.
Language eng
Pub Type(s) Journal Article
PubMed ID 19609880
Source: A qualitative and quantitative HPTLC densitometry method for the analysis of cannabinoids in Cannabis sativa L
Author(s) Fischedick JT, Glas R, Hazekamp A, Verpoorte R
Institution Division of Pharmacognosy, Institute of Biology, Leiden University, Leiden, The Netherlands. jtfische@gmail.com
Source Phytochem Anal 2009 Sep-Oct; 20(5):421-6.
MeSH Cannabinoids
Cannabis
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Densitometry
Flowers
Linear Models
Molecular Structure
Reproducibility of Results
Species Specificity
Tetrahydrocannabinol
Abstract INTRODUCTION: Cannabis and cannabinoid based medicines are currently under serious investigation for legitimate development as medicinal agents, necessitating new low-cost, high-throughput analytical methods for quality control.
OBJECTIVE: The goal of this study was to develop and validate, according to ICH guidelines, a simple rapid HPTLC method for the quantification of Delta(9)-tetrahydrocannabinol (Delta(9)-THC) and qualitative analysis of other main neutral cannabinoids found in cannabis.
METHODOLOGY: The method was developed and validated with the use of pure cannabinoid reference standards and two medicinal cannabis cultivars. Accuracy was determined by comparing results obtained from the HTPLC method with those obtained from a validated HPLC method.
RESULTS: Delta(9)-THC gives linear calibration curves in the range of 50-500 ng at 206 nm with a linear regression of y = 11.858x + 125.99 and r(2) = 0.9968.
CONCLUSION: Results have shown that the HPTLC method is reproducible and accurate for the quantification of Delta(9)-THC in cannabis. The method is also useful for the qualitative screening of the main neutral cannabinoids found in cannabis cultivars.
Language eng
Pub Type(s) Journal Article
PubMed ID 19609880
Source: A qualitative and quantitative HPTLC densitometry method for the analysis of cannabinoids in Cannabis sativa L
